Storeallstocksat4degC,exceptLEU(storeatRT) Letflaskcoolto50degC,thenadd1.0gof5-FOA.100XYC(7DropoutAminoAcidLiquid)
1.0garginine0.5gasparticacid0.5gisoleucine0.5gphenylalanine0.5gproline0.5gserine1.0gthreonine0.5gvalineinfinalvolumeof100mlstirwithlowheatuntilintosolutionfiltersterilze10XYNB+tyrosine
17gYNB(w/oaminoacidsandw/oammoniumsulfate)50gammoniumsulfate0.5gtyrosineinafinalvolumeof1LstirwithlowheatuntilintosolutionfiltersterilzeAminoAcidSolutions(100Xstocks,exceptURA=10Xstock)
URA1mg/mlADE1mg/mlLYS10mg/mlMET5mg/mlTRP10mg/mlLEU10mg/mlHIS5mg/mlAutoclavetosterilize,exceptfiltersterilizeTRPExampleRecipeofCompletePlates
20gofAgarin630mldistilledwaterina2LflaskAutoclave20minutesAddthefollowing:100ml 20%sterileglucose 100ml 10XYNBsolution 10ml 100XYC-7aadropoutsolution 100ml URAsolution 10ml ADEsolution 10ml LYSsolution 10ml METsolution 10ml TRPsolution 10ml LEUsolution 10ml HISsolution FOAplates
20gofAgarin710mldistilledwaterina2LflaskAutoclave20minutesAddthefollowing:100ml 20%sterileglucose 100ml 10XYNBsolution 10ml 100XYC-7aadropoutsolution 20ml URAsolution 10ml ADEsolution 10ml LYSsolution 10ml METsolution 10ml TRPsolution 10ml LEUsolution 10ml HISsolution
