Looking to isolate EVs from Tissue Culture Media or other biofluids? Check out ExoQuick-TC ULTRA.

ExoQuick ULTRA vs. Top Competitor vs. Ultracentrifugation

Figure 1. ExoQuick ULTRA delivers high yields of clean exosomes. (A) A coomassie blue-stained protein gel comparing the protein content of exosome preps isolated using different methods shows only a few, defined protein bands in the ExoQuick ULTRA lane compared to the other methods. (B) Western blotting of the gel shows that the ExoQuick ULTRA prep contains the highest levels of exosome-specific markers CD9, CD81, and Hsp70 and the lowest levels of the carryover proteins albumin and IgGH. In contrast, the prep from Company Q appears to be primarily albumin, and even the sample prepared using ultracentrifugation contains considerably higher levels of both albumin and IgGH. Each lane was loaded with 7 μg of total protein as measured using a fluorometric Qubit protein assay.

Figure 2. Fluorescent nanoparticle tracking analysis (fNTA) demonstrates the high EV yields delivered by ExoQuick ULTRA compared to Ultracentrifugation. Comparison of different isolation methods on EV yields by both volume of input serum (per mL, A) and amount of input serum protein (per mg as measured by fluorometric Qubit protein assay, B). Particle number was measured using fNTA, a technique which specifically detects EVs (see how to use fNTA to measure EV concentration at our ExoGlow-NTA page).

Benefits of ExoQuick ULTRA

• Even cleaner—reduces carry-over of albumins by 75% and immunoglobulins by 40% more than other methods, including ultracentrifugation and competitors’ kits
• Higher yields—isolate more EVs per normalized input volume than ultracentrifugation and competitors’ kits
• Better biomarker detection—see what you’ve been missing when you increase the sensitivity of EV biomarker detection by up to 11-fold (CD9) and 8-fold (CD81)
• Fast—requires less than 20-minutes of hands-on time, including a convenient column-based format for clean-up
• Cost-effective—save money with each reaction compared to how much you’ll spend using a competitor’s kit

4 Simple Stages - 20 Minutes Hands-On-Time

Uncompromising EV isolation delivers higher yields and cleaner preps

Drawing upon our years of exosome experience, the SBI team has pushed ExoQuick extracellular vesicle (EV) isolation technology to new peaks of performance with ExoQuick ULTRA for Serum & Plasma. While many EV isolation methods require you to choose between high yields, easy protocols, clean preps, and low costs, ExoQuick ULTRA is able to deliver on all these fronts for trade-off free EV preparation.

Important for intercellular communication in both normal physiology as well as disease states such as cancer, EV biology is rapidly growing field. However, obtaining EVs for in vivo and ex vivo studies can be challenging. Ultracentrifugation has been considered the gold standard for exosome isolation, but the method is time-consuming, requires large sample volume inputs and requires access to specialized equipment. Additionally, UC doesn’t isolate the cleanest exosome preparations it once was thought to produce. Other commercial kit methods are faster and easier than ultracentrifugation, but still include carryover protein that can cause over-estimation of EV amount and that can interfere with protein-sensitive studies such as mass spectrometry.

Fortunately, the SBI team is never content with good enough and has continued to improve our ExoQuick technology. Now, with our newest generation of ExoQuick—ExoQuick ULTRA—you no longer have to make the trade-off between yield, purity, speed, a simple protocol, and price. From as little as 250 μL of serum or plasma and our ExoQuick ULTRA kit, you can isolate high-quality EVs for a wide range of downstream applications such as such as western blotting, mass spectrometry, NGS sequencing, exosome labeling, and in vivo/ex vivo exosome delivery.

Each ExoQuick ULTRA Kit for Serum and Plasma comes with enough ExoQuick ULTRA (serum/plasma) reagent and convenient, pre-packed columns to complete 20 reactions*.

*1 reaction is defined as 250 μL of serum or plasma precipitated using ExoQuick ULTRA

Figure 3. EVs isolated using ExoQuick ULTRA display typical EV morphology. Transmission electron micrographs of EVs isolated from human serum using ExoQuick. The same sample is shown at two different magnifications. Multiple vesicles with typical EV morphology can be seen in each image.

Want more information on ExoQuick ULTRA? Contact us and a specialist will reach out to you within 24 hours, or speak to a specialist now at 650-968-2200.

SystemBiosciences，简称SBI，美国加利福尼亚湾区新成立的生技公司，致力于独特，创新生物技术之开发，以研发利于基因及蛋白质功能鉴定，研究之崭新方法和工具为宗旨。

美国SBI代理SystemBiosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。现阶段研发重心为RNA干扰（RNAi）研究之相关工具。

现阶段研发重心为RNA干扰（RNAi）的研究之相关工具。系统Biosciences公司（SBI）致力于开发独特，革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具.SBI是专业的慢病毒产品公司，提供基于慢病毒的所有相关产品，质粒，试剂盒及相关配套试剂和慢病毒扩展产品，如IPS细胞多功能性诱导试剂盒和RNAi筛选文库。System Biosciences继续创造新的独特产品以及改善我们完善的产品线。我们对提供领先技术的承诺与我们所有研究试剂和研究项目服务的高质量制造和质量控制相匹配。  2009年要寻找的东西：以下是即将推出的新产品。新型miRZips™：基于慢病毒载体的新型技术可永久敲低MicroRNA。双标记shRNA表达载体：SBI将发布新的功能强大的shRNA表达慢病毒载体pGreenPuro™，以便为稳定的RNAi实验选择稳定转导的细胞的GFP和Puro标记。甾醇反应pGreenFire™慢病毒报告子：基于慢病毒载体的转录报告子，用于监测与固醇感应转录因子相关的转录网络活性-心血管疾病途径的关键。诱导型表达慢载体：新的构建体将允许CDNA，shRNA和microRNA的“按需”表达。2007–2008年的新产品新的miR-SNaRE：MicroRNA小型非编码RNA富集系统，该系统使用带有表位标签的microRNA加工因子来提取蛋白质及其相关RNA。识别驱动RISC复合体的信使RNA。新的GeneNet™聚焦的shRNA库：这些聚焦的shRNA库编码一组针对所有与人类激酶，磷酸酶或细胞凋亡相关基因有关的特定功能或类别基因设计的shRNA集合。这些文库可进行有针对性的高通量RNAi筛选。新的miRNomeMicroRNA分析仪：qPCR阵列在预先格式化的板中包括microRNA分析，可用于人类的完全互补或小鼠单个microRNA的完全互补，每块板上带有三个内源参考RNA对照。所有基于SangermiRBasemicroRNA数据库的microRNA分析均已注册。新的Lenti-miR™MicroRNA前体克隆：在基于HIV的慢病毒载体中可获得更多的microRNA前体集合。超过580个单独的microRNA前体克隆可用阵列形式或合并的慢病毒形式用于HT筛选。新的基于慢病毒的干细胞报道者：SBI越来越多的慢病毒载体已被开发为将基因构建体在体内外几乎传递给任何细胞类型的最有效方法。SBI已将我们的慢病毒构建体系列扩展为干细胞报道分子。使用连接到GFP报告基因的细胞和途径特异性启动子，轻松创建转基因细胞系以监测细胞分化。QuantiMir™RT试剂盒：这项流行的新技术可通过一次cDNA合成同时进行实时qPCR定量分析数百种microRNA。设计您自己的microRNA测定法以进行创新性实验。癌症microRNAqPCR分析小组（OncoMir系列）：预格式化的microRNA分析小组，用于评估95种已知与癌症有关的microRNA。干细胞microRNA分析小组：介绍了95种参与干细胞分化的microRNA，可同时监测干细胞的自我维持，造血途径和神经分化。SBI完善的产品线FullSpectrum™完整信使RNA扩增试剂盒：利用针对mRNA最常见基序的mRNA特异性引物提供完整的mRNA转录物（5"和3"末端）的无偏见，完整代表。GeneNet™siRNA库：全基因组，即用型，预包装的慢病毒库为筛选与生物学反应相关的基因功能提供了令人兴奋的机会。干扰素反应检测试剂盒：区分真正的RNA干扰和压力相关的细胞反应。PathNet™转录报告基因慢病毒载体：一种独特的方法，可创建各种稳定的报告基因细胞系，用于信号通路的研究。我们感谢您过去的支持，并期待为您提供最佳的新试剂，技术和服务，以加速您成功的研究目标。