Description
The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample. QuickExtract Solution has been used to extract DNA from samples such as hair follicles, quill-end cells of feathers, tissue-culture cells, buccal cells, zebrafish organs and scales, and mouse tail snips. The extracted DNA is suitable for PCR analysis, such as genomic, transgenic, or viral DNA screening in animals, or for genetic or environmental research and screening in humans and other organisms.
The QuickExtract method allows for the inexpensive processing of one to hundreds of samples simultaneously, without centrifugation, spin columns or the use of any toxic organic solvent. The method is also compatible with robotic automation.
IMPORTANT NOTE FOR QUICKEXTRACT USERS:
All end-users should bear in mind that QuickExtract Solutions generate crude extracts for PCR. As such many types of PCR inhibitors contained in the original sample will also be present in the crude extract and it is essential to fully control for false negative results in any downstream assays. Furthermore, it is debatable whether all possible types of PCR inhibition can be controlled for adequately using any previously described controls.
It is important to note that BioSearch Technologies/Lucigen/Epicentre QuickExtract Solutions (QuickExtract DNA and QuickExtract RNA) supplied by Cambio Ltd are not validated for use in diagnostic testing (including for SARS-CoV-2). LGC Biosearch and Cambio Ltd take no responsibility for any results obtained. QuickExtract solutions are authorized for Research Purposes ONLY.
Resources
Rapid RNA extraction protocols for SARS-Cov-2 detection
Cui Lab - Washington University - Rapid QuickExtract method
Feng Zhang Lab, Broad Institute – Rapid QuickExtract method
Latest Poster available
QuickExtract – Rapid and efficient extraction of PCR-ready genomic DNA from plant and seed samples
| Figure 1. Procedure for obtaining PCR-ready DNA using QuickExtract™ DNA Extraction Solution. |
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| Figure 2. FailSafe™ PCR amplifications of genomic DNA extracted from a variety of tissues or cells. Buccal cells were extracted using the BuccalAmp™ DNA Extraction Kit, and all other samples with QuickExtract™ DNA Extraction Solution. PCR was performed using primers to amplify the regions indicated: Lanes 1-3, human β-globin; lane 4, transgenic mouse GAPDH; lane 5, E. coli 16S ribosomal RNA gene; lane 6, transgenic SV40 T antigen. |
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| Figure 3. Extracted DNA from multiple Zebrafish organs using QuickExtract™ DNA Extraction Solution 1.0. A 1-µl aliquot of a 100-µl extracted sample was used to amplify a single-copy crystallin-like gene. Lane 1, 100-bp ladder; lanes 2-3, fins; lanes 4-5, eyes; lanes 6-7, scales; lane 8, no-DNA control. |
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