Intact Genomics (IG) i7^® High-Fidelity DNA Polymerase 2x master mix is ready to use premix which contains i7 high-fidelity DNA Polymerase, dNTPs, MgCl₂, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7 high fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has high fidelity, sensitivity and processivity with an error rate ~2.8×10²-fold lower than Taq DNA polymerase, and significantly lower than other proofreading enzymes in the marketplace (1). This 2x master mix product is supplied with 5x magic enhancer that enables efficient amplification of GC rich templates up to 84%.

Activity DataWe have tested i7^® high-fidelity 2x master mix with difficult templates for PCR amplification. Typical PCR results are shown below:

Applications

• Long and difficult template DNA amplification
• Cloning
• High-fidelity PCR
• Efficient for amplifying high GC content template DNA with magic enhancer.

 Product Includes

• i7^® high-fidelity DNA Polymerase 2x master mix
• 5x Magic Enhancer

Storage Temperature–20ºC

 Heat InactivationNo

 Storage Buffer50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC3281 3283

Technical Support

Intact Genomics is committed to supporting the worldwide scientific research community by supplying the highest quality reagents. Each new lot of our products is tested to ensure they meet the quality standards and specifications designated for the product. Please follow the instructions carefully and contact us if additional assistance is needed. We appreciate your business and your feedback regarding the performance of our products in your applications.