The Monarch RNA Cleanup Columns (50 µg) are a component of the Monarch RNA Cleanup Kit (50 µg) (NEB #T2040) and can be used to purify up to 50 µg of RNA from enzymatic reactions including DNase treatment (NEB #M0303), labeling, and capping. The columns are ideal for cleaning up RNA after in vitro transcription reactions such as sgRNA synthesis reactions (NEB #E3322). The columns are designed without the use of a retaining ring, ensuring no buffer retention and no carryover of contaminants. RNA can be eluted in as little as 20 μl for and is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and transfection.
Monarch RNA Cleanup Columns are also available for 10 µg (NEB #T2037) and 500 µg (NEB #T2057) binding capacities.
Figure 1: The Monarch RNA Cleanup Kit enables efficient recovery of RNA in as little as 20 µl
rRNA (50 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch RNA Cleanup Kit (50 µg, NEB #2040) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A260, as measured using a Trinean DropSense 16. Approximately 80% of RNA can be efficiently recovered in as little as 6 µl.Figure 2: The Monarch RNA Cleanup Kit (50 µg) produces sgRNA yields consistent with other competitor RNA cleanup kits and with lower residual NTP contamination
Six different sgRNA synthesis reactions from the EnGen® sgRNA Synthesis Kit, S. pyogenes (NEB #E3322) were cleaned up using either the Monarch RNA Cleanup Kit (50 µg, NEB #T2040) or a competitor kit (according to manufacturer’s recommendations) and eluted with 50 µl of nuclease-free water. sgRNA yield was calculated from the resulting A260, measured using a Trinean Dropsense 16. The Monarch Cleanup Kit produced sgRNA yields consistent with other commercially available RNA cleanup kits.
This product is related to the following categories:
RNA Cleanup Products,
RNA Reagents Products,
RNA Extraction & Purification Products,
Nucleic Acid Purification Products
This product can be used in the following applications:
核糖核酸酶A是内切核糖核酸酶,可特异地攻击RNA上嘧啶残基的3'端,切割与相邻核苷酸形成的磷酸二酯键。反应终产物是嘧啶3'磷酸及末端带嘧啶3'磷酸的寡核苷酸。无辅因子及二价阳离子存在时,核糖核酸酶A的作用可被胎盘RNA酶抑制剂(B1ackburn et al.1977)或氧钒—核糖核苷复合物(Puskas et al.1982)所抑制。核糖核酸酶A用途生化研究,测定核酸的结构RNase 保护检测去除非... 查看更多>
DNase I 是一种核酸内切酶,降解双链或单链DNA,产生5-磷酸末端的单核苷酸及寡核苷酸,在Mg2+存在时,DNase I独立地作用每条DNA链,切割位点是随机分布,在Mn2+存在下,DNase I 作用于DNA双链的大致同一位置,产生钝末端或具1-2个核苷酸突起的DNA片段。来源:牛胰贮存条件:4℃应用:1、用切口平移法进行放射性标记时,可用DNase I在双链DNA上产生随机切口;2、在进行亚硫酸氢盐介导的诱变前,可用DNase... 查看更多>