This package includes 50 nmol of CLIP-Cell 505 substrate, sufficient to make 10 ml of a 5 µM CLIP-tag fusion protein labeling solution.
The CLIP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a protein tag based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether bond. Although CLIP-tag is based on the same protein as SNAP-tag, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal and complementary labeling of two proteins simultaneously in the same cells.
There are two steps to using this system: subcloning and expression of the protein of interest as a CLIP-tag fusion with the CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. The labeling of the fusion proteins is described in this document. Figure 1:Live CHO-K1 cells transiently transfected with pCLIP-NK1RCells were labeled with CLIP-Cell 505 (green) for 60 minutes and counterstained with Hoechst 33342 (blue).Figure 2:Excitation (dotted line) and emission spectra of label 505 coupled to CLIP-tag in buffer at pH 7.5Figure 3:Structure of CLIP-Cell 505 (MW 586.6 g/mol)
This product is related to the following categories:
CLIP-tag™ Substrates Products,
Protein Labeling Products
This product can be used in the following applications:
核糖核酸酶A是内切核糖核酸酶,可特异地攻击RNA上嘧啶残基的3'端,切割与相邻核苷酸形成的磷酸二酯键。反应终产物是嘧啶3'磷酸及末端带嘧啶3'磷酸的寡核苷酸。无辅因子及二价阳离子存在时,核糖核酸酶A的作用可被胎盘RNA酶抑制剂(B1ackburn et al.1977)或氧钒—核糖核苷复合物(Puskas et al.1982)所抑制。核糖核酸酶A用途生化研究,测定核酸的结构RNase 保护检测去除非... 查看更多>
DNase I 是一种核酸内切酶,降解双链或单链DNA,产生5-磷酸末端的单核苷酸及寡核苷酸,在Mg2+存在时,DNase I独立地作用每条DNA链,切割位点是随机分布,在Mn2+存在下,DNase I 作用于DNA双链的大致同一位置,产生钝末端或具1-2个核苷酸突起的DNA片段。来源:牛胰贮存条件:4℃应用:1、用切口平移法进行放射性标记时,可用DNase I在双链DNA上产生随机切口;2、在进行亚硫酸氢盐介导的诱变前,可用DNase... 查看更多>