Product Name | c-Jun(Phospho-Thr91) Antibody |
Host Species | Rabbit |
Clonality | Polyclonal |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Applications | WB IHC |
Reactivity | Hu Ms Rt |
Specificity | The antibody detects endogenous level of c-Jun only when phosphorylated at threonine 91. |
Immunogen | Peptide-KLH. Peptide sequence around phosphorylation site of threonine 91 (T-T-T(p)-P-T) derived from Human c-Jun. |
Target Name | c-Jun |
Modification | Phospho-Thr91 |
Alternative Names | AH119; AP1; Jun A; c-Jun; p39 |
SDS-PAGE MW | 43kd |
Accession No | P05412; NP_002219.1 |
Concentration | 1.0mg/ml |
Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage | Store at -20°C for long term preservation (recommended). Store at 4°C for short term use. |
Application
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Background
Background
Transcription factor that recognizes and binds to the enhancer heptamer motif 5′-TGA[CG]TCA-3′.
Binetruy B, et al. (1991) Nature. 351: 122-127. Smeal T, et al. (1991) Nature. 354:494-496. Derijard B, et al. (1994) Cell. 76:1025-1037. Kyriakis J M, et al. (1994) Nature. 369: 156-160.
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我们使用了南京建成的胆固醇，甘油三酯和游离脂肪酸的试剂盒，样本为小鼠肝脏，试剂盒提供的步骤为使用生理盐水或者乙醇作为匀浆介质，3500rpm,10min;
然而我们发现生理盐水作为匀浆介质时,按此离心速度无法获得上清液；若加大离心速度以及延长离心时间，获得的上清液为白色，蛋白浓度检测出来浓度很高，效果十分不理想；若改为乙醇作为匀浆介质时，检测结果甘油三酯很高，游离脂肪酸浓度很低，不知是否涉及脂肪酯化的问题；
希望大家能给我提提建议，给点自己的妙招；或者是否有人提供好用的试剂盒？
1.我利用微乳法制备SLN，模型药物、三棕榈酸甘油酯、甘油、泊洛沙姆188，80度下融化，混合均匀，2000rpm搅拌状态下滴加1%PVA水相。冷却至室温时，表面有一层脂质析出。不断增加Lipid：surfacant的比例，当达到1:20时，当冷却至室温时，仍有脂质析出。
新人一枚，想问下各位大侠，有什么好的解决方案不？
在兔子上研究肝脏脂代谢，不知道什么细胞株合适，自己培养的担心不靠谱，不知道有没有做过这方面的，给提一些意见，感谢！
