CLIA Kit for Erythropoietin (EPO)
EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin
- Product No.SCA028Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range2.74-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.25pg/mL.
- Sample TypeTissue homogenates, cell lysates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 588 For more details, please contact local distributors!US$ 840 For more details, please contact local distributors!US$ 3780 For more details, please contact local distributors!US$ 7140 For more details, please contact local distributors!US$ 58800 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the CLIA Kit for Erythropoietin (EPO)
This assay has high sensitivity and excellent specificity for detection of Erythropoietin (EPO).No significant cross-reactivity or interference between Erythropoietin (EPO) and analogues was observed.
Precision of the CLIA Kit for Erythropoietin (EPO)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Erythropoietin (EPO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Erythropoietin (EPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Stability of the CLIA Kit for Erythropoietin (EPO)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Erythropoietin (EPO)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;8. Read RLU value immediately.
Test principle of the CLIA Kit for Erythropoietin (EPO)
The microplate provided in this kit has been pre-coated with an antibody specific to Erythropoietin (EPO). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Erythropoietin (EPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Erythropoietin (EPO) level in the sample or standard.;
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Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| APA028Hu61 | Active Erythropoietin (EPO) | Cell culture; Activity Assays. |
| EPA028Hu61 | Eukaryotic Erythropoietin (EPO) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA028Hu01 | Recombinant Erythropoietin (EPO) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA028Hu01 | Active Erythropoietin (EPO) | Cell culture; Activity Assays. |
| PAA028Hu06 | Polyclonal Antibody to Erythropoietin (EPO) | WB; IHC; ICC; IP. |
| PAA028Hu01 | Polyclonal Antibody to Erythropoietin (EPO) | WB; IHC; ICC; IP. |
| LAA028Hu71 | Biotin-Linked Polyclonal Antibody to Erythropoietin (EPO) | WB; IHC; ICC. |
| MAA028Hu22 | Monoclonal Antibody to Erythropoietin (EPO) | WB; IHC; ICC; IP. |
| SEA028Hu | ELISA Kit for Erythropoietin (EPO) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA028Hu | CLIA Kit for Erythropoietin (EPO) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA028Hu01 | ELISA Kit DIY Materials for Erythropoietin (EPO) | Main materials for "Do It (ELISA Kit) Yourself". |
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1.我利用微乳法制备SLN，模型药物、三棕榈酸甘油酯、甘油、泊洛沙姆188，80度下融化，混合均匀，2000rpm搅拌状态下滴加1%PVA水相。冷却至室温时，表面有一层脂质析出。不断增加Lipid：surfacant的比例，当达到1:20时，当冷却至室温时，仍有脂质析出。
新人一枚，想问下各位大侠，有什么好的解决方案不？
在兔子上研究肝脏脂代谢，不知道什么细胞株合适，自己培养的担心不靠谱，不知道有没有做过这方面的，给提一些意见，感谢！
我们使用了南京建成的胆固醇，甘油三酯和游离脂肪酸的试剂盒，样本为小鼠肝脏，试剂盒提供的步骤为使用生理盐水或者乙醇作为匀浆介质，3500rpm,10min;
然而我们发现生理盐水作为匀浆介质时,按此离心速度无法获得上清液；若加大离心速度以及延长离心时间，获得的上清液为白色，蛋白浓度检测出来浓度很高，效果十分不理想；若改为乙醇作为匀浆介质时，检测结果甘油三酯很高，游离脂肪酸浓度很低，不知是否涉及脂肪酯化的问题；
希望大家能给我提提建议，给点自己的妙招；或者是否有人提供好用的试剂盒？
