- Description
- Additional
Description
Details
Description:Mouse monoclonal antibody to human prothymosin alpha (PTMA)
Purification:Protein G affinity purified
Product Type: Primary antibody
Target Protein:Human prothymosin alpha (PTMA)
Immunogen:A short peptide (eeaengrdapangnan) derived from PTMA was conjugated with KLH for immunization.
Fusion Myeloma:Sp2/0-Ag14
Specificity:This antibody is reactive to PTMA peptide (eeaengrdapangnan) and GST tagged recombinant PTMA protein.
Species Reacitvity:Human, others not tested
Host / Isotype:Mouse, IgG2a Kappa
Formulation:Lyophilized from a solution in 0.01M PBS, pH 7.0
Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.
Storage: Store at -20oC
Research Area:Chromatin-remodelling, cell proliferation, oncology
Background:
PTMA (prothymosin alpha) is the precursor of thymosin α1. It is an acidic, non-histone nuclear protein with 109-111 amino acids. PTMA is implicated with cell cycle regulation, chromatin remodeling, expression of oxidative stress-response genes and immuno-modulation. PTMA is elevated in malignances such as breast cancer, gastric cancer, prostate and bladder cancers. PTMA is a potential biomarker for the prognosis of cancer treatment.
Application:
Western blot (see image): This antibody is reactive to GST tagged 38.4KD recombinant PTMA protein.
The GST tagged recombinant PTMA was loaded at 200ng/lane. The anti-PTMA clone 8H12 was used at 1μg/ml and the HRP conjugated goat anti-mouse IgG was used at 1:2,000 dilution.
ELISA: This antibody is reactive to recombinant PTMA protein.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-Human PTMA, 8H12 |
|---|---|
| Application | WB, EIA |
| Size | 0.1 mg |
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1.我利用微乳法制备SLN，模型药物、三棕榈酸甘油酯、甘油、泊洛沙姆188，80度下融化，混合均匀，2000rpm搅拌状态下滴加1%PVA水相。冷却至室温时，表面有一层脂质析出。不断增加Lipid：surfacant的比例，当达到1:20时，当冷却至室温时，仍有脂质析出。
新人一枚，想问下各位大侠，有什么好的解决方案不？
在兔子上研究肝脏脂代谢，不知道什么细胞株合适，自己培养的担心不靠谱，不知道有没有做过这方面的，给提一些意见，感谢！
我们使用了南京建成的胆固醇，甘油三酯和游离脂肪酸的试剂盒，样本为小鼠肝脏，试剂盒提供的步骤为使用生理盐水或者乙醇作为匀浆介质，3500rpm,10min;
然而我们发现生理盐水作为匀浆介质时,按此离心速度无法获得上清液；若加大离心速度以及延长离心时间，获得的上清液为白色，蛋白浓度检测出来浓度很高，效果十分不理想；若改为乙醇作为匀浆介质时，检测结果甘油三酯很高，游离脂肪酸浓度很低，不知是否涉及脂肪酯化的问题；
希望大家能给我提提建议，给点自己的妙招；或者是否有人提供好用的试剂盒？
