| Name | Alpha-factor | ||||||
| Synonyms | α-Factor Mating Pheromone, MFα1 | ||||||
| Sequence | WHWLQLKPGQPMY | ||||||
| 3-letter-code | Trp - His - Trp - Leu - Gln - Leu - Lys - Pro - Gly - Gln - Pro - Met - Tyr | ||||||
| Molecular weight | 1684.00 | ||||||
| Counter ion | TFA | ||||||
| References | K Jeppsson, K.K. Carlborg, R. Nakato, D. G. Berta, I. Lilienthal, T. Kanno, A. Lindqvist, M. C. Brink, N. P. Dantuma, Y. Katou, K. Shirahige, C. Sj�gren.The Chromosomal Association of the Smc5/6 Complex Depends on Cohesion and Predicts the Level of Sister Chromatid Entanglement.PLOS Genetics 2014. DOI 10.1371/journal.pgen.1004680A Kegel, H Betts-Lindroos, T Kanno,K Jeppsson,L Str�m, Y Katou,T Itoh, K Shirahige& C Sjogren.Chromosome length influences replication-induced topological stress.Nature 2011 471,392�396H. Betts-Lindroos, L Strom, T. Itoh, K. Shirahige, and C. Sjogren.Chromosomal association of the Smc5/6 complex reveals that it functions in differently regulated pathways.Mol. Cell. 2006;22;755-767. | ||||||
Order Alpha-factor peptide
| Amount | Cat.No. | Unit price | |||||
|---|---|---|---|---|---|---|---|
| 1 mg | SP-AFMP-1 | 75 EUR | BUY | ||||
| 10 mg | SP-AFMP-10 | 330 EUR | BUY | ||||
| 50 mg | SP-AFMP-50 | 660 EUR | BUY | ||||
| For larger amounts please inquire. | |||||||
Alpha-factor antibody
Please inquire for pricing and availability.ebiomall.com
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我们使用了南京建成的胆固醇,甘油三酯和游离脂肪酸的试剂盒,样本为小鼠肝脏,试剂盒提供的步骤为使用生理盐水或者乙醇作为匀浆介质,3500rpm,10min;
然而我们发现生理盐水作为匀浆介质时,按此离心速度无法获得上清液;若加大离心速度以及延长离心时间,获得的上清液为白色,蛋白浓度检测出来浓度很高,效果十分不理想;若改为乙醇作为匀浆介质时,检测结果甘油三酯很高,游离脂肪酸浓度很低,不知是否涉及脂肪酯化的问题;
希望大家能给我提提建议,给点自己的妙招;或者是否有人提供好用的试剂盒?
在兔子上研究肝脏脂代谢,不知道什么细胞株合适,自己培养的担心不靠谱,不知道有没有做过这方面的,给提一些意见,感谢!
1.我利用微乳法制备SLN,模型药物、三棕榈酸甘油酯、甘油、泊洛沙姆188,80度下融化,混合均匀,2000rpm搅拌状态下滴加1%PVA水相。冷却至室温时,表面有一层脂质析出。不断增加Lipid:surfacant的比例,当达到1:20时,当冷却至室温时,仍有脂质析出。
新人一枚,想问下各位大侠,有什么好的解决方案不?

