![Anaspec/[Cys(HiLyteᵀᴹ Fluor 647 C2 maleimide)] - Exendin - 4/50 ug/AS-63714](images/Anaspec/icon_msds.gif)
Product Name | [Cys(HiLyteᵀᴹFluor 647 C2 maleimide)] - Exendin - 4C(HiLyteFluor 647 C2 maleimide) - HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPS - NH2 |
Size | 50 ug |
Catalog # | AS-63714 |
US$ | $118 |
Purity | % Peak Area By HPLC ≥ 95% |
This peptide is HiLyte Fluor 647 labeled Extendin-4 (Ex/Em=650 nm/675 nm).A cysteine residue has been added to the peptide N-terminus, and the dye is conjugated to this cysteine via the cysteine thiol moiety. Exendin-4, an agonist of glucagon-like peptide 1(GLP-1) receptor, induces release of insulin after food intake.Exendin-4 shares a 53% sequence homology with GLP-1. Derived from Gila monster, Heloderma suspectum, Exendin-4 has a longer half life than GLP-1 in the plasma, thus making it a more potent insulinotropic agent. | |
Detailed Information | ![]() |
Storage | -20°C |
References | Eng, J. et al. J Biol Chem 267, 7402 (1992)Greig, NH. et al. Diabetolog 42, 45 (1999)Göke R. et al. J Biol Chem 268, 19650 (1993) |
Molecular Weight | 5486.3 |
C(HiLyte Fluor 647 C2 maleimide)-HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 | |
Sequence(Three-Letter Code) | H - Cys(Hilyte Fluor 647 C2 maleimide) - His - Gly - Glu - Gly - Thr - Phe - Thr - Ser - Asp - Leu - Ser - Lys - Gln - Met - Glu - Glu - Glu - Ala - Val - Arg - Leu - Phe - Ile - Glu - Trp - Leu - Lys - Asn - Gly - Gly - Pro - Ser - Ser - Gly - Ala - Pro - Pro - Pro - Ser - NH2 |
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我们使用了南京建成的胆固醇,甘油三酯和游离脂肪酸的试剂盒,样本为小鼠肝脏,试剂盒提供的步骤为使用生理盐水或者乙醇作为匀浆介质,3500rpm,10min;
然而我们发现生理盐水作为匀浆介质时,按此离心速度无法获得上清液;若加大离心速度以及延长离心时间,获得的上清液为白色,蛋白浓度检测出来浓度很高,效果十分不理想;若改为乙醇作为匀浆介质时,检测结果甘油三酯很高,游离脂肪酸浓度很低,不知是否涉及脂肪酯化的问题;
希望大家能给我提提建议,给点自己的妙招;或者是否有人提供好用的试剂盒?
1.我利用微乳法制备SLN,模型药物、三棕榈酸甘油酯、甘油、泊洛沙姆188,80度下融化,混合均匀,2000rpm搅拌状态下滴加1%PVA水相。冷却至室温时,表面有一层脂质析出。不断增加Lipid:surfacant的比例,当达到1:20时,当冷却至室温时,仍有脂质析出。
新人一枚,想问下各位大侠,有什么好的解决方案不?
在兔子上研究肝脏脂代谢,不知道什么细胞株合适,自己培养的担心不靠谱,不知道有没有做过这方面的,给提一些意见,感谢!

