
Product Name | SensoLyte ® 490 MMP - 7 Assay Kit *Fluorimetric* |
Size | 1 kit |
Catalog # | AS-71132 |
US$ | $631 |
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known. MMP-7 (matrilysin, PUMP) is proposed as a potential anticancer drug target. The SensoLyte® 490 MMP-7 Assay Kit is a complete assay system designed to continuously analyze MMP activities or to screen MMP-7 inhibitors by using a fluorogenic MMP-7 substrate. The assays are performed in a convenient 96-well or 384-well microplate format. The kit provides speed, convenience and accuracy in analyzing MMP-7 activities and screening MMP-7 inhibitors. Kit size: 500 assays | |
Detailed Information | ![]() ![]() |
Storage | -20°C |
Product Citations | Nishihara, S. et al. (2008). Evaluation of the expression and enzyme activity of matrix metalloproteinase-7 in fetal membranes during premature rupture of membranes at term in humans.Repro. Sci. 15, 156. doi: 10.1177/1933719107310308. |
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我们使用了南京建成的胆固醇,甘油三酯和游离脂肪酸的试剂盒,样本为小鼠肝脏,试剂盒提供的步骤为使用生理盐水或者乙醇作为匀浆介质,3500rpm,10min;
然而我们发现生理盐水作为匀浆介质时,按此离心速度无法获得上清液;若加大离心速度以及延长离心时间,获得的上清液为白色,蛋白浓度检测出来浓度很高,效果十分不理想;若改为乙醇作为匀浆介质时,检测结果甘油三酯很高,游离脂肪酸浓度很低,不知是否涉及脂肪酯化的问题;
希望大家能给我提提建议,给点自己的妙招;或者是否有人提供好用的试剂盒?
1.我利用微乳法制备SLN,模型药物、三棕榈酸甘油酯、甘油、泊洛沙姆188,80度下融化,混合均匀,2000rpm搅拌状态下滴加1%PVA水相。冷却至室温时,表面有一层脂质析出。不断增加Lipid:surfacant的比例,当达到1:20时,当冷却至室温时,仍有脂质析出。
新人一枚,想问下各位大侠,有什么好的解决方案不?
在兔子上研究肝脏脂代谢,不知道什么细胞株合适,自己培养的担心不靠谱,不知道有没有做过这方面的,给提一些意见,感谢!

