
INFORMATION: The p75 low affinity human Nerve Growth Factor receptor CD271(NGRF, TNFRSF16) is expressed on a variety of tissue types including a subset of splenic and nodal lymphocytes. Presence of this receptor supports uptake of intracellular calcium, but not mobilization (3). CD271 is a stem cell marker for hematopoietic(5,6,7), stromal (8) as well as cancer cells (9,10). CD271 has been reported to inhibit differentiation of mesenchymal cells into various lineages(11).
Molecular Structure: A soluble fusion protein consisting of the extracellular (195aa) domain of human NGFR fused to murine IgG2a Fc (233aa). N-teminal sequence: (29)KEACP
Transfectant Cell Line: CHO :
Immunochemistry Applications: NGFR-muIg binds to coated NGFbeta in ELISA.
References:
1) Brodie C, E W Gelfand (1992) Journal of Immunology. 148(11): 3492-3497.
2) Fantini F, O. Johansson (1992) Journal of Investigative Dermatology. 99(6): 734-742.
3) Jiang H, G Gurogg, et al.(1999) J Biol Chem 274(37): 26209-16.
4) Khwaja F, D Djakiew, et al (2004) Cancer Res 64(17): 6207-13.
5) Flores-Torales E, Cuneo-Pareto S, et al. (2010) Folia Histochem Cytobiol. 48(4):682-6.
6) Kuçi S, Bader P, et al. (2010) Haematologica. 95(4):651-9.
7) Hermida-Gómez T, Blanco FJ, et al. (2011) Tissue Eng Part A. 17(7-8):1169-79. 8) Jones E, McGonagle D, et al. (2010) Arthritis Rheum. 62(7):1944-54.
9) Boiko AD, Weissman IL. Et al. (2010) Nature 466(7302):133-7.
10) Civenni G, Sommer L, et al. (2011) Cancer Res. 71(8):3098-109.
11) Mikami Y, Honda MJ, et al. (2011) Stem Cells Dev. 20(5):901-13.
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受体是细胞在进化过程中形成的细胞蛋白组分,能识别周围环境中某种微量化学物质,首先与之结合,并通过中介的信息转导与放大系统,触发随后的生理反应或药理效应。自从Langley 提出受体学说100年后,受体已被证实为客观存在的实体,类型繁多,作用机制多已被阐明,现在受体已不再是一个空泛笼统的概念。受体分子在细胞中含量极微,1mg 组织一般只含10fmol左右。能与受体特异性结合的物质称为配体(ligand)。受体仅是一个“感觉器”,对相应配体有极高的识别能力。受体-配体是生命活动中的一种偶合,受体都有其内源性配体,如神经递质、激素、自身活性物(autocoid)等。能激活受体的配体称为激动药(agonist),能阻断其活性的配体称为拮抗药(antagonist)。根据受体与配体结合的高度特异性,受体被分为若干亚型,如肾上腺素受体又分为α1、α2、β1和β2等亚型,其分布及功能都有区别。受体与配体有高度亲和力,多数配体在1pmol~1nmol/L的浓度时即可引起细胞的药理效应。反应之所以如此灵敏主要是靠后续的信息转导系统,如细胞内第二信使(second messenger)的放大、分化及整合功能。酶、载体、离子通道及核酸也可与药物直接作用,但这些物质本身具有效应力,故严格地说不应被认为是受体。某些细胞蛋白组分可与配体结合,但没有触发效应的能力,称为结合体(acceptor)。

