GenCatch TM Gel Extraction Kit
| Catalog# | size | Price | Quantity | |
|---|---|---|---|---|
| 2260050 | 50 Mini Preps | $45.00 | ||
| 2260250 | 250 Mini Preps | $185.00 |
Description | GenCatch TM Gel Extraction Kit purifies DNA fragments from agarose gel electrophoresis.Recovery efficiency of DNA fragments ranging from 70 bp to 10 kb in size is up to80%. It totally eliminate slurry carryover problem as encountered using silica beadsor glass milk based methods. Up to 10 ¦Ìg DNA can be recovered with a single columnand elution volumes can be as little as 10 ¦Ìl. | ||||
Detailed Components | Mini-spin columns, collection tubes together with buffers and protocols. | ||||
Features | Capacity: Up to 10 ¦Ìg of DNA. Elution Volume: 10-50¦Ìl. Recover Efficiency: Up to 80%. Quality: DNA purified using GenCatch TM Gel Extraction Kit is free from agraose,carry-over slurry and salt contamination. And is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformationand transfection, radioactive and fluorescent sequencing. Fast:Single samplescan be prepared within 10 minutes with minimum efforts. 18-24 samples canbe simultaneously processed in a centrifugation format within 30 minutes. Or, morecan be processed using a vacuum manifold. Flexible: Mini columnscan used in either centrifugation procedure or using a vacuum manifold. Convenient: No phenolextractions or ethanol precipitations required. | ||||
Technical Data | PCR products of various MW (lane 1-5) were purified using GenCatch TM PCRCleanup Kit and loaded as a cocktail (lane 6) onto 1.5% agraose gel. Recovery efficiencycan be roughly estimated by comparing the overall intensity of the individual bands. | ||||
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现在有一个实验需要看一种药物与受体的结合情况,想咨询一下放射性配体受体结合实验,因为我们不具备这个实验条件,最好能外包那种
受体是细胞在进化过程中形成的细胞蛋白组分,能识别周围环境中某种微量化学物质,首先与之结合,并通过中介的信息转导与放大系统,触发随后的生理反应或药理效应。自从Langley 提出受体学说100年后,受体已被证实为客观存在的实体,类型繁多,作用机制多已被阐明,现在受体已不再是一个空泛笼统的概念。受体分子在细胞中含量极微,1mg 组织一般只含10fmol左右。能与受体特异性结合的物质称为配体(ligand)。受体仅是一个“感觉器”,对相应配体有极高的识别能力。受体-配体是生命活动中的一种偶合,受体都有其内源性配体,如神经递质、激素、自身活性物(autocoid)等。能激活受体的配体称为激动药(agonist),能阻断其活性的配体称为拮抗药(antagonist)。根据受体与配体结合的高度特异性,受体被分为若干亚型,如肾上腺素受体又分为α1、α2、β1和β2等亚型,其分布及功能都有区别。受体与配体有高度亲和力,多数配体在1pmol~1nmol/L的浓度时即可引起细胞的药理效应。反应之所以如此灵敏主要是靠后续的信息转导系统,如细胞内第二信使(second messenger)的放大、分化及整合功能。酶、载体、离子通道及核酸也可与药物直接作用,但这些物质本身具有效应力,故严格地说不应被认为是受体。某些细胞蛋白组分可与配体结合,但没有触发效应的能力,称为结合体(acceptor)。
大多数药物在体内都是和特异性受体相互作用,改变细胞的生理生化功能而产生效应。目前已经确定的受体有30多种,根据受体存在的标准,受体可大致分为三类:
1.细胞膜受体:位于靶细胞膜上,如胆碱受体、肾上腺素受体、多巴胺受体、阿片受体等。
2.胞浆受体:位于靶细胞的胞浆内,如肾上腺皮质激素受体、性激素受体。
3.胞核受体:位于靶细胞的细胞核内,如甲状腺素受体。
另外也可根据受体的蛋白结构、信息转导过程、效应性质、受体位置等特点将受体分为四类:
1.含离子通道的受体(离子带受体):如N-型乙酰胆碱受体含钠离子通道。
2.G蛋白偶联受体:M-乙酰胆碱受体、肾上腺素受体等。
3.具有酪氨酸激酶活性的受体:如胰岛素受体。
4.调节基因表达的受体(核受体):如甾体激素受体、甲状腺激素受体等。
有些受体具有亚型,各种受体都有特定的分布部位核特定的功能,有些细胞也有多种受体。
请教各位大神,我最近在做WB,565KD的蛋白,动物组织的样品,听说样品制备需要蔗糖裂解液,请问这个是必须的嘛?用一般蛋白提取方法制备可以吗?还有电泳和转膜条件能否分享一下。不胜感激


PCR products of various MW (lane 1-5) were purified using GenCatch TM PCRCleanup Kit and loaded as a cocktail (lane 6) onto 1.5% agraose gel. Recovery efficiencycan be roughly estimated by comparing the overall intensity of the individual bands.