Product Specifications:
Item# 1021-P: Peroxidase-Conjugated rgp120 HIV-1 MN
Concentration: See vial
Mass/vial: 25µg
Volume/vial: 100µl
Diluent: 50mM Tris, pH 8 1% BSA
Preservative: 0.01% Thimerasol
Storage: 4°C
Stability: At least 3 months at 4°C
Minimum Dilution: 1 : 1000
Application: ELISA/Western ELISA, Human T-cell Research, Histocytochemistry, Drug Screening.
Description: Peroxidase conjugated full length rgp120 HIV-1 MN.
Molar Ratio: 2 - 4 peroxidase molecules per molecule of gp120.
Specificity: Peroxidase conjugated rgp120 MN binds to murine monoclonal antibodies of defined epitope specificity in ELISA and Western ELISA
Biological Activity: Peroxidase conjugated rgp120 MN binds to immobilized human T-cell receptor CD4 in ELISA and Western ELISA.
Application and Instructions for use
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
1. ELISA and Western ELISA may be performed at dilutions of 1 : 2000 depending on the concentration of CD4 in the test system. Binding to murine and human polyclonal antibodies may be performed at higher dilutions.
Item#: 1021 Full Length rgp120 HIV-1 MN
Application: Human diagnostics, CD4 binding, Drug screening, Human CD4 T-cell studies.
Item#: 1021-G Colloidal gold-Conjugated rgp120 HIV-1 MN
Application: Human diagnostics, CD4 binding, Drug screening, Histocytology.
Item#: 1021-F Flourescene-Conjugated rgp120 HIV-1 MN
Application: FACS analysis, Histocytology.
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现在有一个实验需要看一种药物与受体的结合情况，想咨询一下放射性配体受体结合实验，因为我们不具备这个实验条件，最好能外包那种
大多数药物在体内都是和特异性受体相互作用，改变细胞的生理生化功能而产生效应。目前已经确定的受体有30多种，根据受体存在的标准，受体可大致分为三类：
1．细胞膜受体：位于靶细胞膜上，如胆碱受体、肾上腺素受体、多巴胺受体、阿片受体等。
2．胞浆受体：位于靶细胞的胞浆内，如肾上腺皮质激素受体、性激素受体。
3．胞核受体：位于靶细胞的细胞核内，如甲状腺素受体。
另外也可根据受体的蛋白结构、信息转导过程、效应性质、受体位置等特点将受体分为四类：
1．含离子通道的受体（离子带受体）：如N-型乙酰胆碱受体含钠离子通道。
2．G蛋白偶联受体：M-乙酰胆碱受体、肾上腺素受体等。
3．具有酪氨酸激酶活性的受体：如胰岛素受体。
4．调节基因表达的受体（核受体）：如甾体激素受体、甲状腺激素受体等。
有些受体具有亚型，各种受体都有特定的分布部位核特定的功能，有些细胞也有多种受体。
