Product Specifications: Item# 2501-B: Biotin-Conjugated Human Anti-gp120 HIV-1 pAb IgG
Mass/vial: 100µg
Volume: 100µl
Diluent: PBS, 0.1% BSA
Stabilizer: 0.05% Azide
Preservative: 0.02% Na-Z
Storage: 4°C
Stability: At least 3 months at 4°C
Minimum Dilution: 1 : 500
Applications: ELISA, Westerns, Immunohistocytology, gp120 Capture Assays, Drug Screening, Human T-cell Research
Description: Biotin conjugated Human anti-gp120 HIV-1 pAb (IgG).
Molar Ratio: 2 - 4 Biotin molecules per molecule of pAb.
Specificity: Biotin conjugated Human anti-gp120 pAb binds to recombinant gp120 in ELISA and Western ELISA.
Biological Activity: Biotin conjugated Human anti-gp120 pAb binds to gp120 in gp120 Capture ELISA and Western ELISA.
Application and Instructions for use: Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA and Western ELISA may be performed at dilutions of 1 : 500 depending on the concentration of GP120 in the test system. gp120/GP120 capture ELISA may be performed in 1 : 500 dilution range depending on the capture ligand gp120 concentration and GP120 concentration in the test sample. |
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现在有一个实验需要看一种药物与受体的结合情况,想咨询一下放射性配体受体结合实验,因为我们不具备这个实验条件,最好能外包那种
大多数药物在体内都是和特异性受体相互作用,改变细胞的生理生化功能而产生效应。目前已经确定的受体有30多种,根据受体存在的标准,受体可大致分为三类:
1.细胞膜受体:位于靶细胞膜上,如胆碱受体、肾上腺素受体、多巴胺受体、阿片受体等。
2.胞浆受体:位于靶细胞的胞浆内,如肾上腺皮质激素受体、性激素受体。
3.胞核受体:位于靶细胞的细胞核内,如甲状腺素受体。
另外也可根据受体的蛋白结构、信息转导过程、效应性质、受体位置等特点将受体分为四类:
1.含离子通道的受体(离子带受体):如N-型乙酰胆碱受体含钠离子通道。
2.G蛋白偶联受体:M-乙酰胆碱受体、肾上腺素受体等。
3.具有酪氨酸激酶活性的受体:如胰岛素受体。
4.调节基因表达的受体(核受体):如甾体激素受体、甲状腺激素受体等。
有些受体具有亚型,各种受体都有特定的分布部位核特定的功能,有些细胞也有多种受体。

