| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Mouse IgG1 Kappa |
| Purification : | Protein G Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
| Gene : | IKBKB |
| Gene ID : | 3551 |
| Uniprot ID : | O14920 |
| Alternative Name : | IKBKB, IKKB |
| Immunogen Information : | Full length recombinant Ikkb protein was used as the immunogen for this antibody. |
IKKB also known as inhibitor of nuclear factor kappa-B kinase subunit beta is a protein which is encoded by the IKBKB (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta) gene. IKKB causes dissociation of the inhibitor and activation of NF-kappa-B which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Higher expression of this protein is found in liver, kidney and spleen.
Western blot analysis: 2-4 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Nucleus, Membrane raft |
| Post transnational modification: | Hydroxylated by PHD1/EGLN2, loss of hydroxylation under hypoxic conditions results in activation of NF-kappa-B. |
| Tissue Specificity: | Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood. |
| BioGrid: | 109767.151 interactions. |
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请教各位大神,我最近在做WB,565KD的蛋白,动物组织的样品,听说样品制备需要蔗糖裂解液,请问这个是必须的嘛?用一般蛋白提取方法制备可以吗?还有电泳和转膜条件能否分享一下。不胜感激

