
Recombinant Human UbcH5b/UBE2D2 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-622
Formulation | Xmg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: UbcH5b/UBE2D2
Ubiquitin-conjugating Enzyme H5b (UbcH5b), also known as Ubiquitin-conjugating Enzyme E2D 2 (UBE2D2), is a widely expressed member of the Ubiquitin-conjugating (E2) enzyme family (1). The protein has a predicted molecular weight of 16.5 kDa. UbcH5b/UBE2D2 localizes to both the nucleus and the cytoplasm (2). The human protein shares 100% and 92% amino acid sequence identity with the mouse and rat orthologs, respectively. This enzyme has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (3). UbcH5b/UBE2D2 is capable of mediating the formation of Ubiquitin chains linked through Lys11, Lys48, or Lys63 (4). Working with the SCF(Fbxw2) and MDM2/HDM2 Ubiquitin ligases (E3s), UbcH5b/UBE2D2 mediates the ubiquitination and degradation of the transcription factors GCM1 and p53, respectively (5-7). Along with UBE2N/Ubc13, UbcH5b/UBE2D2 may have a role in the endocytosis and endolysosomal degradation of MHC class I molecules (8). Non-proteolytic ubiquitination of TRIM5-alphaby UbcH5b/UBE2D2 has been reported to block HIV reverse transcription (9). Pathologically, UBE2D family members may be critical targets of cadmium during cadmium-induced renal toxicity (10). Additionally, overexpression of UbcH5b/UBE2D2 has been linked to inflammatory bowel disease (11).
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- Umebayashi, K. et al. (2008) Mol. Biol. Cell 19:3454.
- Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
- Ordureau, A. et al. (2008) Biochem. J. 409:43.
- Chiang, M.H. et al. (2008) Biol. Reprod. 79:914.
- Saville, M.K. et al. (2004) J. Biol. Chem. 279:42169.
- Iyappan, S. et al. (2010) J. Biol. Chem. 285:33065.
- Duncan, L.M. et al. (2006) EMBO J. 25:1635.
- Yamauchi, K. et al. (2008) FEBS J. 275:1540.
- Tokumoto, M. et al. (2011) J. Toxicol. Sci. 36:191.
- Hetzenecker, A.M. et al. (2012) Digestion 86:34.
Citations for Recombinant Human UbcH5b/UBE2D2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
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- Mitochondria ubiquitin ligase, MARCH5 resolves hepatitis B virus X protein aggregates in the liver pathogenesisAuthors: YS Yoo, YJ Park, HS Lee, NTK Oanh, MY Cho, J Heo, ES Lee, H Cho, YY Park, H ChoCell Death Dis, 2019;10(12):938.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15Authors: TC Ting, M Goralski, K Klein, B Wang, J Kim, Y Xie, D NijhawanCell Rep, 2019;29(6):1499-1510.e6.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- The E3 ubiquitin ligase Siah-1 suppresses avian reovirus infection by targeting p10 for degradationAuthors: X Chen, Z He, M Fu, Y Wang, H Wu, X Li, H Cao, SJ ZhengJ. Virol., 2018;0(0):.Applications: Bioassay
- RNF126 Quenches RNF168 Function in the DNA Damage ResponseAuthors: L Zhang, Z Wang, R Shi, X Zhu, J Zhou, B Peng, X XuGenomics Proteomics Bioinformatics, 2018;0(0):.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- COP1 mediates dark-specific degradation of microtubule-associated protein WDL3 in regulating Arabidopsis hypocotyl elongationAuthors: N Lian, X Liu, X Wang, Y Zhou, H Li, J Li, T MaoProc. Natl. Acad. Sci. U.S.A., 2017;114(46):12321-12326.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disabilityAuthors: M Bosshard, R Aprigliano, C Gattiker, V Palibrk, E Markkanen, P Hoff Backe, S Pellegrino, FL Raymond, G Froyen, M Altmeyer, M Bjørås, GL Dianov, B van LoonSci Rep, 2017;7(1):15050.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- The Banana Fruit SINA Ubiquitin Ligase MaSINA1 Regulates the Stability of MaICE1 to be Negatively Involved in Cold Stress ResponseAuthors: ZQ Fan, JY Chen, JF Kuang, WJ Lu, W ShanFront Plant Sci, 2017;8(0):995.Applications: Bioassay
- Reciprocal proteasome-mediated degradation of PIFs and HFR1 underlying photomorphogenic development in ArabidopsisAuthors: X Xu, PK Kathare, VN Pham, Q Bu, A Nguyen, E HuqDevelopment, 2017;0(0):.Applications: Bioassay
- BLADE-ON-PETIOLE proteins act in an E3 ubiquitin ligase complex to regulate PHYTOCHROME INTERACTING FACTOR 4 abundanceAuthors: B Zhang, M Holmlund, S Lorrain, M Norberg, L Bakó, C Fankhauser, O NilssonElife, 2017;6(0):.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- Epilepsy-associated gene Nedd4-2 mediates neuronal activity and seizure susceptibility through AMPA receptorsAuthors: J Zhu, KY Lee, KA Jewett, HY Man, HJ Chung, NP TsaiPLoS Genet, 2017;13(2):e1006634.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor TurnoverAuthors: R Tawo, W Pokrzywa, É Kevei, ME Akyuz, V Balaji, S Adrian, J Höhfeld, T HoppeCell, 2017;169(3):470-482.e13.Applications: Bioassay
- Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradationAuthors: X Wei, X Wang, J Zhan, Y Chen, W Fang, L Zhang, H ZhangJ. Cell Biol., 2017;0(0):.Applications: Bioassay
- Phosphorylation of CHIP at Ser20 by Cdk5 promotes tAIF-mediated neuronal death.Authors: Kim C, Yun N, Lee J, Youdim M, Ju C, Kim W, Han P, Oh YCell Death Differ, 2016;23(2):333-46.Species: N/ASample Types: ProteinApplications: Enzyme Assay
- Identification and Characterization of MCM3 as a Novel KEAP1 SubstrateJ Biol Chem, 2016;0(0):.Species: HumanSample Types: ProteinApplications: Ubiquitination
- Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewalNat Commun, 2016;7(0):12326.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay
- 14-3-3? loss leads to activation of the epithelial to mesenchymal transition due to the stabilization of c-JunAuthors: Kumarkrishna RaychaudhuJ Biol Chem, 2016;0(0):.Species: HumanSample Types: ProteinApplications: Bioassay
- PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxiaAuthors: RS Banh, C Iorio, R Marcotte, Y Xu, D Cojocari, AA Rahman, J Pawling, W Zhang, A Sinha, CM Rose, M Isasa, S Zhang, R Wu, C Virtanen, T Hitomi, T Habu, SS Sidhu, A Koizumi, SE Wilkins, T Kislinger, SP Gygi, CJ Schofield, JW Dennis, BG Wouters, BG NeelNat. Cell Biol, 2016;18(7):803-13.Species: HumanSample Types: Cell LysatesApplications: Ubiquitination
- Fine-tuning of ULK1 mRNA and protein levels is required for autophagy oscillationAuthors: Francesca NazioJ. Cell Biol, 2016;215(6):841-856.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- Vaccinia-related kinase 2 mediates accumulation of polyglutamine aggregates via negative regulation of the chaperonin TRiC.Authors: Kim, Sangjune, Park, Do-Young, Lee, Dohyun, Kim, Wanil, Jeong, Young-Hu, Lee, Juhyun, Chung, Sung-Kee, Ha, Hyunjung, Choi, Bo-Hwa, Kim, Kyong-TaMol Cell Biol, 2014;34(4):643-52.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- The E3 ubiquitin ligases RNF126 and Rabring7 regulate endosomal sorting of the epidermal growth factor receptor.Authors: Smith, Christop, Berry, Donna M, McGlade, C JaneJ Cell Sci, 2013;126(0):1366-80.Species: N/ASample Types: ProteinApplications: Bioassay
- Characterization of ML-IAP protein stability and physiological role in vivo.Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, DomagojBiochem J, 2012;447(3):427-36.Species: HumanSample Types: ProteinApplications: Bioassay
- DNA damage-induced heterogeneous nuclear ribonucleoprotein K sumoylationregulates p53 transcriptional activation.Authors: Pelisch F, Pozzi B, Risso G, Munoz M, Srebrow AJ Biol Chem, 2012;287(36):30789-99.Species: N/ASample Types: ProteinApplications: Bioassay
- The F-box protein FBXO44 mediates BRCA1 ubiquitination and degradation.Authors: Lu, Yunzhe, Li, Jiezhi, Cheng, Dongmei, Parameswaran, Balaji, Zhang, Shaohua, Jiang, Zefei, Yew, P Renee, Peng, Junmin, Ye, Qinong, Hu, YanfenJ Biol Chem, 2012;287(49):41014-22.Species: N/ASample Types: Recombinant ProteinApplications: Ubiquitination
- HDM2 antagonist MI-219 (spiro-oxindole), but not Nutlin-3 (cis-imidazoline), regulates p53 through enhanced HDM2 autoubiquitination and degradation in human malignant B-cell lymphomas.Authors: Sosin, Angela M, Burger, Angelika, Siddiqi, Aisha, Abrams, Judith, Mohammad, Ramzi M, Al-Katib, Ayad MJ Hematol Oncol, 2012;5(0):57.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay
- Functional analysis of NopM, a novel E3 ubiquitin ligase (NEL) domain effector of Rhizobium sp. strain NGR234.Authors: Xin DW, Liao S, Xie ZPPLoS Pathog., 2012;8(5):e1002707.
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大多数药物在体内都是和特异性受体相互作用,改变细胞的生理生化功能而产生效应。目前已经确定的受体有30多种,根据受体存在的标准,受体可大致分为三类:
1.细胞膜受体:位于靶细胞膜上,如胆碱受体、肾上腺素受体、多巴胺受体、阿片受体等。
2.胞浆受体:位于靶细胞的胞浆内,如肾上腺皮质激素受体、性激素受体。
3.胞核受体:位于靶细胞的细胞核内,如甲状腺素受体。
另外也可根据受体的蛋白结构、信息转导过程、效应性质、受体位置等特点将受体分为四类:
1.含离子通道的受体(离子带受体):如N-型乙酰胆碱受体含钠离子通道。
2.G蛋白偶联受体:M-乙酰胆碱受体、肾上腺素受体等。
3.具有酪氨酸激酶活性的受体:如胰岛素受体。
4.调节基因表达的受体(核受体):如甾体激素受体、甲状腺激素受体等。
有些受体具有亚型,各种受体都有特定的分布部位核特定的功能,有些细胞也有多种受体。
请教各位大神,我最近在做WB,565KD的蛋白,动物组织的样品,听说样品制备需要蔗糖裂解液,请问这个是必须的嘛?用一般蛋白提取方法制备可以吗?还有电泳和转膜条件能否分享一下。不胜感激

