DescriptionViPrimePLUS One Step Taq RT-qPCR Green Master Mix II is next generation master mix designed for one step real-time PCR reaction set up. The master mix is prepared in 2X concentrated solution and contains unique thermostable M-MULV enzyme, Taq DNA Polymerases, EvaGreen® dye, as well as MgCl2 and buffer components at optimal concentrations. The MMULV enzyme has an optimal operating temperature and a higher affinity for primer template duplexes which allows very rapid processing during RT step. EvaGreen® dye in master mix is environmentally safe and highly stable which can be formulated with relative high dye concentration to maximize fluorescence signal without PCR inhibition.ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The formulation of RT-qPCR master mix can detect low copy number targets very specifically with high efficiency that give CT values close to the theoretical time of detection. The ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II is a complete system for use in one step real-time PCR, the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The master mix provides convenient and robust set up for quantitative real-time analysis of RNA samples.ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II has several formulations optimized to be used with most of real-time PCR instruments. The sensitivity and consistency of ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II in standard cycling conditions gives the industry leading performance in fast cycling conditions.
ApplicationsAll kinds of RNA sample material suited for RT-qPCR amplification can be used.
Features
- One step real time RT-qPCR reaction set up
- Equipped with thermostable M-MULV enzyme EvaGreen® dye – highest dye stability and safety
- Good buffer system for excellent amplification efficiency
- Minimal PCR inhibition
- Reliable quantification of low copy number targets
- Minimal PCR inhibition
- Optimal performance for highly sensitive and specific RT-qPCR reaction
- Compatible with most of the real-time PCR platforms
Component3 x 0.6ml aliquots of master mix 0.6ml aliquots of “no RT control master mix standard”
StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.
Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS Taq qPCR Green Master Mix II has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.
Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.
InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.
| Product Description | Compatible Hardware |
| QLMM18ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II (EvaGreen® Dye) | Biometra qTower, BioRad iCycler, BioRad IQ4, BioRad IQ5, Cepheid SmartCycler®, Eppendorf Mastercycler, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® 480, lightcycler® LC96 and lightcycler® Nano Platforms, RotorGene, Roche Capillary Lightcycler 1.0-2.0, Stratagene MX MX4000P®, MX3000P®, MX3005®, Thermo PikoReal™ |
| QLMM18-LRViPrimePLUS One Step Taq RT-qPCR Green Master Mix II with Low ROX (EvaGreen® Dye) | Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7 |
| QLMM18-RViPrimePLUS One Step Taq RT-qPCR Green Master Mix II with ROX (EvaGreen® Dye) | Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT FAST platforms, GeneAmp® 5700, StepOne™, StepOne™ PLUS |
Ordering Information
| Catalog No | Description | Pack Size |
| QLMM18 | ViPrimePLUS One Step Taq RT-qPCR Green MasterMix II (EvaGreen® Dye) | 150 reactions |
| QLMM18-LR | ViPrimePLUS One Step Taq RT-qPCR Green MasterMix II with Low ROX (EvaGreen® Dye) | 150 reactions |
| QLMM18-R | ViPrimePLUS One Step Taq RT-qPCR Green MasterMix II with ROX (EvaGreen® Dye) | 150 reactions |
DownloadManual
ViPrimePLUS One Step Taq RT-qPCR Green MasterMix II (EvaGreen® Dye) ViPrimePLUS One Step Taq RT-qPCR Green MasterMix II with Low ROX (EvaGreen® Dye) ViPrimePLUS Taq qPCR Green MasterMix II with ROX (EvaGreen® Dye)
PublicationThis Product Has Been Used In: Jahantigh, M., Mazaheri, M., Moghadam, A.Y., Motalleb, G., Najafi, S., Najafi, S., Pourrahmat, E., Rashki, A., Sabagh, K., Sanadgol, N., Talee, R. (2014).Epidermal growth factor receptor gene expression evaluation in colorectal cancer patients, Indian Journal of Cancer, Vol. 51, No. 3, 358-362 (2014).
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大多数药物在体内都是和特异性受体相互作用，改变细胞的生理生化功能而产生效应。目前已经确定的受体有30多种，根据受体存在的标准，受体可大致分为三类：
1．细胞膜受体：位于靶细胞膜上，如胆碱受体、肾上腺素受体、多巴胺受体、阿片受体等。
2．胞浆受体：位于靶细胞的胞浆内，如肾上腺皮质激素受体、性激素受体。
3．胞核受体：位于靶细胞的细胞核内，如甲状腺素受体。
另外也可根据受体的蛋白结构、信息转导过程、效应性质、受体位置等特点将受体分为四类：
1．含离子通道的受体（离子带受体）：如N-型乙酰胆碱受体含钠离子通道。
2．G蛋白偶联受体：M-乙酰胆碱受体、肾上腺素受体等。
3．具有酪氨酸激酶活性的受体：如胰岛素受体。
4．调节基因表达的受体（核受体）：如甾体激素受体、甲状腺激素受体等。
有些受体具有亚型，各种受体都有特定的分布部位核特定的功能，有些细胞也有多种受体。
请教各位大神，我最近在做WB，565KD的蛋白，动物组织的样品，听说样品制备需要蔗糖裂解液，请问这个是必须的嘛？用一般蛋白提取方法制备可以吗？还有电泳和转膜条件能否分享一下。不胜感激
