iLite ADCC Target mTNF-alpha Negative Assay Ready Cells
iLite ADCC Target mTNF-alpha Negative Assay Ready Cells Developed and Manufactured by Svar Life Science
The Eagle Biosciences iLite ADCC Target mTNF-alpha (-) Assay Ready Cells are intended for testing:
- ADCC activity
- anti-mTNF-alpha ADCC activity
Content: >250 μL of Assay Ready Cells suspended in cryoprotective medium from Gibco
Receipt and Storage: Upon receipt confirm that adequate dry-ice is present, and the cells are frozen. Immediately transfer to -80°C storage. Cells should be stored at -80°C (do not store at any other temperature) and are stable as supplied until the expiry date shown. Cells should be used within 30 min of thawing.
For Research Use Only
Key benefits of iLite® Assays
- Highly specific reporter gene cell lines
- Very sensitive cell line responses (>10 fold inductions)
- Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
- Assays within a workday (typically 4-7 hour assays)
- Normalization gene, which eliminates unwanted matrix effects
Description
iLite® ADCC Target mTNF-alpha- Assay Ready Cells are based on a human embryonic kidney cell line, HEK293, and have been genetically engineered and optimized to deplete all expression of the surface protein mTNF-alpha. The cells are for use as internal controls together with iLite® ADCC Effector (V) Assay Ready Cells and iLite® ADCC Target mTNF-alpha (+) Assay Ready Cells when measuring the ADCC activity of anti-TNF-alpha antibodies.
Background
Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism whereby pathogenic cells are lysed by lymphocytes, most often Natural Killer (NK) cells. The mechanism involves binding of antibodies to surface antigens on the pathogen. Crosslinking of these antibodies to NK cells through the binding of the Fc-portion to Fc receptors on the NK cells leads to activation of the NK cell and formation of an immune synapse with the pathogenic cell. The NK cell releases cytotoxic granules containing granzymes and perforin into the synapse, leading to apoptosis of the targeted cell (1).
Related Products
iLite Effector V Assay Ready Cells
iLite ADCC Target mTNF-alpha+
iLite ADCC anti-mTNF-alpha Activity Set
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细胞信号转导分析的生物信息学方法:
a)选择关键词，从GO数据库中寻找相关基因，比如extracellular表示为分泌蛋白
b)通过GO,BioCarta和Kegg信号通路数据分析给定基因所属的信号通路，功能分类等
c)比较多组基因按功能，通路分组在统计学上的差异，从而得到各组基因的功能差异
d)新信号通路的分析
回复：【资源】很有价值的信号通路分析平台方法!-丁香园论坛
pathwaybuild
我最近跑电泳NF-kB信号通路，跑了p65和IkB-α两个蛋白，p65显影的时候有结果，但是IkB-α显示的时候是没有条带，空白一片，我跑电泳的条件不变、蛋白样品也是相同的，但是一个有结果，一个没有结果，请问原因是什么，请大神帮忙解答一下，谢谢啦~~
→MAPKK→MAPK→进入细胞核→其它激酶或基因调控蛋白（转录因子） 的磷酸化修钸。
信号通路的组成： 配体――生长因子； RTK—酪氨酸；
接头蛋白（生长因子受体接头蛋白-2， GRB-2）； GRF－－鸟苷酸释放因子； Ras—GTP 结合蛋白； Raf――是丝氨酸/苏氨酸（Ser/Thr）
蛋白激酶（称 MAPKKK）。
主要功能： 调节细胞的增殖与分化， 促进细胞存活， 以及细胞代谢过程中的调节与校正。
全部的：www.cell.com/snapshots
信号通路1.zip(33848.99k)
信号通路2.zip(24706.36k)
请问哪种神经细胞可以代替原代海马神经元培养细胞？PC12?HT-22?SH-SY5Y?BV-2一般都适用于什么模型，大家一般用哪个呢
大家好,
我是今年刚毕业找到一份实验室研究工作的小猫,以前常作蛋白合成和分子克隆的,新实验室的老板想要研究些信号通路问题,具体就是研究一个病毒的致病途径是什么,定了大方向后我就扎到了文献的海洋中,可是越看就越有一个问题不明白.
就是我能找到的几乎所有文章,都是用的癌细胞做的模型,比如说hela,就是乳头瘤,293,就是肾瘤,还有SH5Y,就是神经瘤.我不太懂,就是病毒感染人的时候,不是感染的正常细胞吗,用病毒来感染癌细胞,然后在这些癌细胞中检测病毒的作用阿，信号通路之类的，要怎么绕过癌细胞其实和正常细胞不一样这个问题呢？
我看大部分文献都不在这方面作解释说明，仿佛都是约定俗成的用癌细胞，我对信号通路研究也是啥都不懂，两眼抹黑，求各路大神赐教ORZ
【2】信号：信号是数据的电磁编码或电子编码。和数据一样，信号也分为模拟信号和数字信号。模拟信号是指电信号的参量是连续取值的，其特点是幅度连续。常见的模拟信号有电话、传真和电视信号等。数字信号是离散的，从一个值到另一个值的改变是瞬时的，就像开启和关闭电源一样。数字信号的特点是幅度被限制在有限个数值之内。常见的数字信号有电报符号、数字数据等。信号是运载消息的工具，是消息的载体。从广义上讲，它包含光信号、声信号和电信号等。
