QuantiQuik™ Malic Acid Quick Test Strips
- Product Overview
- Product FAQ
- Product Citations
- Assay Service


Application
- Direct determination of malic acid concentrations in food and beverage samples as well as biological samples.
Key Features
- Fast and sensitive. Use of 20 or 100 µL sample. Semi-quantitative measurement between 0-500 mg/L (undiluted) malic acid.
- Convenient. No expensive lab equipment needed.
- Sample treatment and assay can be performed in under 15 minutes.
Method
- Visual
Samples
- Wine, beer, milk, etc
Species
- All
Size
- 10 tests
Shelf Life
- 6 months
More Details
- L-MALIC ACID, or L-malate, is a dicarboxylic acid that is made by all living organisms and plays an important role in the Calvin and Krebs Cycle. Malic acid is frequently used in food and beverage industries as an additive in products such as wine, beer, candies, etc. It can be used as an acidity regulator, preservative, or simply added for flavor. In the wine industry, decreasing levels of L-malic acid and increasing levels of L-Lactic Acid are monitored during malolactic fermentation. As L-malic acid is converted to L-lactic acid, the overall acidity of the wine is reduced which can lead to the improvement of the flavor of the wine. BioAssay Systems QuantiQuik™ L-Malic Acid Test Strips are based on L-malate dehydrogenase catalyzed oxidation of L-malic acid in which the formed NADH reduces a chromogenic reagent. The intensity of the product color is directly proportional to the L-malic acid concentration in the sample.
What samples have you tested?
The strips have been tested on red wine, white wine, homogenized whole milk, cultured buttermilk, almond milk, yogurt, beer, apple juice, lemonade, grape juice, orange juice, cranberry juice, lime and lemon juice.My sample turns the strip very dark purple, how can I determine the D-lactate concentration?
Very dark purple indicates that the diluted sample concentration is greater than 500 mg/L. To obtain a more accurate concentration, the sample should be further diluted and retested. For example, if the sample was diluted 5×, try diluting 21×.I don’t have access to a pipetteman. How can I accurately measure out my samples?
We offer exact volume transfer pipettes as an accessory. For samples requiring a 21× dilution, order the 20 µL transfer pipettes (TP20). For samples requiring a 5× dilution, order the 100 µL transfer pipettes (TP100).Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. The strips should be kept in a dry, cool location and protected from light.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.ebiomall.com
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如题,实验小白,但是导师给了这个课题,对于这通路不是很了解,园子里也没找到关于非经典通路的,希望各位战友帮忙下,谢谢了
信号通路图:向左转|向右转
相关抑制剂:Selumetinib(AZD6244)是一种有效,高选择性的MEK1抑制剂,IC50为14 nM,也抑制ERK1/2磷酸化,IC50为10 nM,对p38α, MKK6, EGFR, ErbB2, ERK2, B-Raf等没有抑制作用。Phase 3。Vemurafenib(PLX4032, RG7204)是一种新型有效的B-RafV600E抑制剂,IC50为31 nM。SB203580是一种p38 MAPK抑制剂,IC50为0.3-0.5 μM,与SAPK3(106T)和SAPK4(106T)相比选择性低10倍,且抑制PKB磷酸化,IC50为3-5 μM。参考:www.selleck.cn/pharmacological_MAPK.html
pathwaybuild
全部的:www.cell.com/snapshots
信号通路1.zip(33848.99k)
信号通路2.zip(24706.36k)
通常来说,整条信号通路上下游的关键信号蛋白很多,所以做信号通路变化的话,建议您用高通量的蛋白芯片技术对信号通路磷酸化水平进行检测。针对多条信号通路变化检测,可以选择信号通路磷酸化广谱筛选抗体芯片(PEX100),一次芯片实验即可实现多条信号通路的同步筛选和具体调变位点的清晰定位,为后续生物现象的深入探索提供明确的研究方向。针对单条信号通路,如MAPK,p53, mTOR等,同样有相对应的芯片产品,好像有个华盈生物,专门做蛋白芯片的,你可以咨询一下。 祝好。
大家好,
我是今年刚毕业找到一份实验室研究工作的小猫,以前常作蛋白合成和分子克隆的,新实验室的老板想要研究些信号通路问题,具体就是研究一个病毒的致病途径是什么,定了大方向后我就扎到了文献的海洋中,可是越看就越有一个问题不明白.
就是我能找到的几乎所有文章,都是用的癌细胞做的模型,比如说hela,就是乳头瘤,293,就是肾瘤,还有SH5Y,就是神经瘤.我不太懂,就是病毒感染人的时候,不是感染的正常细胞吗,用病毒来感染癌细胞,然后在这些癌细胞中检测病毒的作用阿,信号通路之类的,要怎么绕过癌细胞其实和正常细胞不一样这个问题呢?
我看大部分文献都不在这方面作解释说明,仿佛都是约定俗成的用癌细胞,我对信号通路研究也是啥都不懂,两眼抹黑,求各路大神赐教ORZ
我最近跑电泳NF-kB信号通路,跑了p65和IkB-α两个蛋白,p65显影的时候有结果,但是IkB-α显示的时候是没有条带,空白一片,我跑电泳的条件不变、蛋白样品也是相同的,但是一个有结果,一个没有结果,请问原因是什么,请大神帮忙解答一下,谢谢啦~~
→MAPKK→MAPK→进入细胞核→其它激酶或基因调控蛋白(转录因子) 的磷酸化修钸。
信号通路的组成: 配体――生长因子; RTK—酪氨酸;
接头蛋白(生长因子受体接头蛋白-2, GRB-2); GRF--鸟苷酸释放因子; Ras—GTP 结合蛋白; Raf――是丝氨酸/苏氨酸(Ser/Thr)
蛋白激酶(称 MAPKKK)。
主要功能: 调节细胞的增殖与分化, 促进细胞存活, 以及细胞代谢过程中的调节与校正。

