| Product Name | SensoLyte ® AFC Caspase Profiling Kit *Fluorimetric* |
| Size | 1 kit |
| Catalog # | AS-71116 |
| US$ | $698 |
Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AFC-based substrates yield blue fluorescence upon protease cleavage. They are widely used to monitor caspase activity. The SensoLyte® Caspase Profiling Kit contains a series of AFC-based peptide substrates (Ex/Em=380 nm/500 nm) as fluorogenic indicators for assaying caspase protease activities. The kit contains a well-designed plate in which a series of AFC-based caspase substrates are coated with both positive and negative controls. It provides the best solution for profiling caspases or caspase inhibitors. The kit contains: A 96-well plate coated with a series of AFC-based caspase substrates along with various controls* Cell lysis buffer Assay buffer AFC (fluorescence reference standard for calibration) A detailed protocol A detailed protocol *384-well plate format is available on a custom basis. Kit Size: 2 plates | |
| Detailed Information | |
| Storage | -20°C |
| Product Citations | Jaiswal, MK. et al. (2013). Regulation of apoptosis and innate immune stimuli in inflammation-induced preterm labor. J Immunol 191, 5702. doi: 10.4049/jimmunol.1301604.Shteingart, S. et al. (2012). Endoplasmic reticulum stress induces a caspase-dependent N-terminal cleavage of RBX1 protein in B cells. J Biol Chem 287, 31223.Lopez, V. et al. (2010). Zip6-attenuation promotes epithelial-to-mesenchymal transition in ductal breast tumor (T47D) cells. Exp Cell Res 316, 366. |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
根据磷酸化的底物不同,可将蛋白激酶分为组蛋白蛋白激酶、酪蛋白蛋白激酶等,但由于蛋白激酶可磷酸化底物的多样化,这种分法很不确切,已经被根据底物磷酸化氨基酸的分类方法所取代,有些如酪蛋白蛋白激酶,只是由于习惯而一直被沿用下来。根据有无调节物将蛋白激酶分为信使依赖的蛋白激酶和非信使依赖的蛋白激酶,有些信使依赖的蛋白激酶的首字母缩略词已为人们所接受,如cAMP依赖的蛋白激酶PKA、钙和磷脂依赖的蛋白激酶PKC以及钙依赖钙调素不依赖的蛋白激酶CDPK等,它们彼此间存在结构和功能上的相关关系。
也有人认为:蛋白激酶在信号转导中主要作用有两个方面:其一是通过磷酸化调节蛋白质的活性,磷酸化和去磷酸化是绝大多数信号通路组分可逆激活的共同机制,有些蛋白质在磷酸化后具有活性,有些则在去磷酸化后具有活性;其二是通过蛋白质的逐级磷酸化,使信号逐级放大,引起细胞反应.
根据PTK是否存在于细胞膜受体可将其分成受体型和非受体型。向左转|向右转
protease
水解蛋白质肽键的一类酶的总称。按其水解多肽的方式,可以将其分为内肽酶和外肽酶两类。内肽酶将蛋白质分子内部切断,形成分子量较小的月示和胨。外肽酶从蛋白质分子的游离氨基或羧基的末端逐个将肽键水解,而游离出氨基酸,前者为氨基肽酶后者为羧基肽酶。按其活性中心和最适pH值,又可将蛋白酶分为丝氨酸蛋白酶、巯基蛋白酶、金属蛋白酶和酸性蛋白酶。按其反应的最适pH值,分为酸性蛋白酶、中性蛋白酶和碱性蛋白酶。工业生产上应用的蛋白酶,主要是内肽酶。
蛋白激酶
protein kinase
又称蛋白质磷酸化酶(protein phosphakinase)。一类催化蛋白质磷酸化反应的酶。
大家好,有几个设计的药物分子需要测试活性,不知道谁能提供下能做相关测试服务的机构或个人信息不?非常感谢!

