Product Name | Human MMP - 1, Proenzyme100 uL, 10ug/mL |
Size | 100 uL |
Catalog # | AS-72004 |
US$ | $98 |
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. The human MMP-1 is purified from fibroblasts and becomes highly active when incubated with APMA. It can be used as a positive control in FRET-based enzyme activity assay, zymography, Western blot, IP, and ELISA. This is sold as a pro-form and activation is required. | |
Detailed Information | DatasheetMaterial Safety Data Sheets (MSDS) |
Product Citations | Bahney, C. et al. (2011). A bioresponsive hydrogel tuned to chondrogenesis of human mesenchymal stem cells. FASEB J doi: 10.1096/fj.10-165514.Salsas-Escat, R. et al. (2010). Cleavage Site Specificity and Conformational Selection in Type I Collagen Degradation. Biochem 49, 4147.Spiga, M. et al. (2010). Development of a Gene Therapy Virus with a Glucocorticoid-Inducible MMP1 for the Treatment of Steroid Glaucoma. Investigat Ophthalmol Visual Sci 51, 3029. |
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肌酸激酶有四种同功酶形式:肌肉型(MM)、脑型(BB)、杂化型(MB)和线粒体型(MiMi)。MM型主要存在于各种肌肉细胞中,BB型主要存在于脑细胞中,MB型主要存在于心肌细胞中,MiMi型主要存在于心肌和骨骼肌线粒体中。肌肉型肌酸激酶分子是由两个相同的亚基组成的二聚体。根据目前已经测定的兔、人、鸡、鼠肌酸激酶的一级结构[3-6],M型亚基由387个氨基酸残基组成,质量为43 KDa左右,分子内有8个巯基,但无二硫键。大熊猫肌肉型肌酸激酶也是二聚体酶,每个亚基由376个氨基酸残基组成,分子量为42 KDa[7]。向左转|向右转
磷酸肌酸激酶(CPK),主要存在于骨骼肌和心肌,在脑组织中也存在,是参与体内的能量代谢的一种酶。在临床上主要用于诊断心肌梗塞。心肌梗塞患者发病后2-4小时,血液中此酶活动即开始升高。比血清中谷草转酸酶和乳酸脱氢酶的活力变化都出现得早。
临床意义:
(1)心肌梗塞后,CPK较谷草转氨酶和乳酸脱氢酶特异性高,但持续时间短,2-4天恢复正常。
(2)病毒性心肌炎,CPK也可升高,对诊断及预后有参考价值。
(3)进行性肌营养不良、多发性肌炎以及肌肉损伤时CPK也可升高。
(4)严重的心绞痛、心包炎、房颤、脑血管意外、脑膜炎以及心脏手术等,CPK可见升高。