Description
Product Highlights
- Fast - streamlined protocol for extraction of eighteen plasmid samples in as little as 15 minutes
- Efficient – rapid SDS/alkaline lysis and efficient column-binding for high-yield recovery of all plasmid molecules
- High-performance – efficient extraction of high-purity plasmid DNA, ideal for use in all downstream applications
- Flexible – reliable recovery of high- and low-copy plasmids from all gram-positive bacteria
Product Description
The ISOLATE II Plasmid Mini Kit provides a simple, efficient column-based method for the isolation of plasmid DNA from bacterial cultures, without the need for hazardous reagents such as phenol.
By combining SDS/alkaline lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Plasmid Mini Kit provides a fast method for the purification of high-quality plasmid DNA. Separate protocols are provided for the isolation of high-copy plasmids, the isolation of low-copy plasmid, P1 constructs and cosmid DNA from E. coli and the isolation of plasmids from gram-positive bacteria such as Bacillus, Staphylococcus, Bifidobacteria or Corynebacteria.
The ISOLATE II Plasmid Mini Kit has been designed to deliver optimal performance in cloning, sequencing as well as end-point PCR alongside any enzyme from the Meridian PCR portfolio, including MyTaq DNA Polymerase. Additionally, the ISOLATE II Plasmid Mini Kit can be used in tandem with the SensiFAST Real-Time PCR Kits for high-performance qPCR.
Applications
- Cloning
- Sequencing
- Restriction digestion
- Labeling
- End-point PCR
- qPCR
- Transfection
- Fluorescent sequencing
- In vitro transcription

Nucleic Acid Isolation Guide
Download the ISOLATE II Guide with detailed product descriptions and performance data to help you choose the best product for your researchISOLATE II Selection Chart
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