Zeatin Riboside is the most active and ubiquitous form of the naturally occurring cytokinins. Cytokinins promote cell division, stimulate shoot proliferation, inhibit root formation, slow the aging process, and activate gene expression and metabolic activity in general. In mature plants, natural cytokinin hormone levels are high during maximum growth periods. Concentrations of cytokinin used for horticulture vary between 0.1 to 10 mg/L.
The plant hormones cytokinins (CKs) comprise a class of growth regulators involved in the stress response, senescence, photosynthesis, nutrient assimilation and mobilization, as well as modulation of a plant tissue’s ability to act as a sink or source of metabolites.
CKs are recognized by a subset of sensor histidine kinases (HKs) and the signal is transmitted to the nucleus via a multistep phosphorelay (MSP), thus activating the expression of target genes.
Product SpecificationsTrans Zeatin Riboside6-[(E)-4-Hydroxy-3-methylbut-2-enylamino]-9-β-D-ribofuranosylpurine
MOLECULAR BIOLOGY GRADE
Formula: C15H21N5O5
MW: 351.37 g/mol
Storage/Handling: Store at -15°C.
PubChem Chemical ID:6440982
Selected ReferencesWerner T, Schmulling T (2009) Cytokinin action in plant development. Curr Opin PlantBiol 12: 527-538.
Hwang I, Sheen J, Muller B (2012) Cytokinin signaling networks. Annual review of plantbiology 63: 353-380.
GoldBio活体成像技术:早在1999年由美国哈佛大学Weissleder博士率先提出了分子影像学(molecularimaging,MI)的概念,即应用影像学的方法对活体状态下的生物过程进行细胞和分子水平的定性和定量研究。活体成像便是基于分子影像学孕育而生的,通过这个成像系统,可以观测活体动物体内肿瘤的生长及转移,感染性疾病的发展进程,特定基因的表达等生物学过程。活体成像技术主要采用生物发光(bioluminescence)与荧光(fluorescence)两种技术。★生物发光是用荧光素酶基因标记细胞或DNA。★荧光技术则采用荧光报告基团(GFP、RFP,Cyt及dyes等)进行标记。★这一技术对肿瘤微小转移灶的检测灵敏度极高,不涉及放射性物质和方法,非常安全。操作极其简单、所得结果直观、灵敏度高。
活体成像两种检测技术介绍活体成像特点优点缺点生物发光检测bioluminescence★荧光素酶(Luciferase)对基因、细胞和活体动物进行标记;★荧光素酶催化底物(例如荧光素钾盐)反应后,会产生化学发光。这种光是由化学反应而来,不需要激发光;★标记方法是通过克隆技术,将荧光素酶的基因插入到预期观察的细胞染色体内,通过对克隆细胞进行筛选,培养出能稳定表达荧光素酶的细胞株。再将细胞株转移至特定的小鼠体内形成模型。★特异性强,无自发荧光;★高灵敏度,在体内可检测到几百个细胞,检测的深度在3-100px;★定量精确 ★信号较弱,检测时间较长;★仪器精密度要求较高;★细胞或基因需要转基因标记;★不可用于人体,不适用于抗体、多肽等标记荧光检测fluorescence★采用荧光报告基因(GFP、RFP等)或荧光染料进行标记;★需要外接激发光源,利用报告基因、荧光蛋白质或染料产生的荧光,就可以形成体内的生物光源。★荧光染料、蛋白标记能力强;★信号强,成像速度快,操作简便,实验成本较低;★未来可用于人;★适用范围广,可以是动物、细胞、微生物,也可以是抗体、药物、纳米材料等。★存在自发荧光,影响灵敏度;★光容易被动物组织吸收;★检测深度受限;★背景光干扰,定量准确度低
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>