下载所得到的文件列表clodronateliposomesimprovemetabolicprofileandreducevisceraladiposemacrophagecontentindiet-inducedobesemiceclodronate脂质体提高新陈代谢和减少内脏脂肪在食源性肥胖小鼠巨噬细胞的内容.pdf
ClodronateLiposomesImproveMetabolicProfileand
ReduceVisceralAdiposeMacrophageContentin
Diet-InducedObeseMice
BinFeng1,3,PingJiao3,YaohuiNie3,ThomasKim3,DaleJun3,NicovanRooijen2,ZaiqingYang1,Haiyan
Xu3*
1KeyLaboratoryofAgriculturalAnimalics,BreedingandReproductionofMinistryofEducation,CollegeofLifeScienceandTechnology,HuazhongAgricultural
University,Wuhan,China,2DepartmentofMolecularCellBiology,FacultyofMedicine,VrijeUniversiteit,Amsterdam,herlands,3AlpertMedicalSchool,Hallett
CenterforDiabetesandEndocrinology,BrownUniversity,Providence,RhodeIsland,UnitedStatesofAmerica
Abstract
Background:Obesity-relatedadiposeinflammationhasbeenthoughttobeacausalfactorforthedevelopmentofinsulin
resistanceandtype2diabetes.Infiltratedmacrophagesinadiposetissueofobeseanimalsandhumansareanimportant
sourceforinflammatorycytokines.Clodronateliposomescanablatemacrophagesbyinducingapoptosis.Inthisstudy,we
aimtodeterminewhetherperitonealinjectionofclodronateliposomeshasanybeneficialeffectonsystemicglucose
homeostasis/insulinsensitivityandwhethermacrophagecontentinvisceraladiposetissuewillbereducedindiet-induced
obese(DIO)mice.
Methodology/PrincipalFindings:ClodronateliposomeswereusedtodepletemacrophagesinleanandDIOmice.
Macrophagecontentinvisceraladiposetissue,metabolicparameters,glucoseandinsulintolerance,adiposeandliver
histology,adipokineandcytokineproductionwereexamined.Hyperinsulinemic-euglycemicclampstudywasalso
performedtoassesssystemicinsulinsensitivity.Peritonealinjectionofclodronateliposomessignificantlyreducedblood
glucoseandinsulinlevelsinDIOmice.Systemicglucosetoleranceandinsulinsensitivityweremildlyimprovedinbothlean
andDIOmicetreatedwithclodronateliposomesbyintraperitoneal(ip)injection.Hepatosteatosiswasdramatically
alleviatedandsuppressionofhepaticglucoseoutputwasmarkedlyincreasedinDIOmicetreatedwithclodronate
liposomes.MacrophagecontentinvisceraladiposetissueofDIOmicewaseffectivelydecreasedwithoutaffecting
subcutaneousadiposetissue.Interestingly,levelsofinsulinsensitizinghormoneadiponectin,includingthehighmolecular
weightform,weresignificantlyelevatedincirculation.
Conclusions/Significance:Intraperitonealinjectionofclodronateliposomesreducesvisceraladiposetissuemacrophages,
improvessystemicglucosehomeostasisandinsulinsensitivityinDIOmice,whichcanbepartiallyattributabletoincreased
adiponectinlevels.
Citation:FengB,JiaoP,NieY,KimT,JunD,etal.(2011)ClodronateLiposomesImproveMetabolicProfileandReduceVisceralAdiposeMacrophageContentin
Diet-InducedObeseMice.PLoSONE6(9):e24358.doi:10.1371/journal.pone.00内容来自淘豆网www.taodocs.com转载请标明出处.