医疗器械领域未来的王者,一定出自高值耗材细分领域! 来源:瞪羚社 导 ...
来自 : 蚂蚁淘
OUTLINEExperimentalsplenectomyinthemouseisusedtostudytheroleofthespleeninplateletdestruction..PROTOCOLGeneralanesthesia
- injecti.p.250µl/mouseoftheANESTHETICmixture<waitfor2-10minfortheanestheticeffect>
- forverifytheanestheticeffectcheckplantarreflexes(animalmustbeunresponsivetosqueezinghindfeet)
Preparationoftheoperationarea1.washthesurgicalandadjacentareawithC2H5OH-70%2.razethefurwiththerazor3.washthesurgicalareawithC2H5OH-70%Operation
- Atthelefthypochondriummakea1.5-2cm"skin,musclesandperitoneum"incisionwithsurgicalscissors
- Gentlytrapandpushthespleenfreeofadjacenttissue,separateitsмlegоwithblunt-endedscissorsorforceps
- Byasingleknottieoffthearterywitha3-0planeCATGUTbyloopingthesuturethroughthemesentary(thearteryentersthespleenatthesuperiorpolus).Ifnecessarybyasingleknottieofftheefferentvenule(exitsthespleenattheinferiorpolus)
- Cutawaytheconnectivetissueandremovethespleen
- Applyontheopenwound1pinchofAstrexene(ChlorhexidinHCl)(5mg/g)
- ApplyontheperitoneumandmusclestheмUо-likesuturewithplaneCATGUT(3-0)
- ApplyontheskintheмUо-likesuturewithETHICONMERSILENE(3-0)
Wakening1.inject250ul/mouseofthe"WAKENINGMIXTURE"Postoperationperiod1.aftertheoperationtookplacelettheanimalstorestforatleast7daysinacagewiththefilter2.observeanimalseverydaySOLUTIONS
- ANESTHETICMIXTURE=mix1mlofDomitor(MedetomidinHCl)(1mg/ml),1mlofImalgene(KetaminumHCl)(50mg/ml)and8mlofPBS<allsteril>.
- "WAKENINGMIXTURE"=mix1mlofANTISEDAN(AtipamezoleHCl)(5mg/ml)and9mlofPBS<allsterile!>
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