Catalog Number:
Source:PolyclonalRabbit
Preparation:ProducedfromseraofrabbitsimmunizedwithhighlypureRecombinantHumansCD14.Anti-HumansCD14-specificantibodywaspurifiedbyaffinitychromatographyemployinganimmobilizedHumansCD14matrix.
Immunogen:HEK293cells derivedRecombinantHumansCD14 (PeproTechcatalog#110-01)
SandwichELISA:TodetectHumansCD14bysandwichELISA(using100μl/wellantibodysolution)aconcentrationof0.5-2.0μg/mlofthisantibodyisrequired.Thisantigenaffinitypurifiedantibody,inconjunctionwithPeproTech’sBiotinylatedAnti-HumansCD14(500-P320Bt)asadetectionantibody,allowsthedetectionofatleast2000-4000pg/mlofrecombinantHumansCD14.
WesternBlot:TodetectHumansCD14byWesternBlotanalysisthisantibodycanbeusedataconcentrationof0.1-0.2μg/ml.UsedinconjunctionwithcompatiblesecondaryreagentsthedetectionlimitforRecombinantHumansCD14is1.5-3.0ng/lane,undereitherreducingornon-reducingconditions.
Note:
Additionalapplicationstestedonalot-to-lotbasis.Please contact TechnicalSupportformoreinformation.
CountryOfOrigin: USA
ResearchUseOnly:Notforuseindiagnosticortherapeuticprocedures.
