

Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.

ThisantibodyisshippedwithitsantigenFREEofcharge!
- Peptide(C)RQPSSPGPQPRNLSE,correspondingtoaminoacidresidues191-205ofmouseα1-syntrophin(Accession Q61234).Intracellular,PH1domain.
- Westernblotanalysisofmouseheartlysate(lanes1and3)andratskeletalmusclemembranes(lanes2and4):1,2. Anti-α1-Syntrophin(SNTA1)Antibody (#APZ-021),(1:400).
3,4.Anti-α1-Syntrophin(SNTA1)Antibody,preincubatedwiththenegativecontrolantigen.Westernblotanalysisofmousebrainlysate:1. Anti-α1-Syntrophin(SNTA1)Antibody (#APZ-021),(1:200).
2.Anti-α1-Syntrophin(SNTA1)Antibody,preincubatedwiththenegativecontrolantigen.
- ExpressionofSyntrophinalpha1inmouseneocortexImmunohistochemicalstainingofperfusion-fixedfrozenmousebrainsectionsusingAnti-α1-Syntrophin(SNTA1)Antibody (#APZ-021),(1:400),followedbyanti-rabbit-AlexaFluor-488antibody(green).Syntrophinalpha1staining(green)appearsinbloodvessels(horizontalarrows)andinthepiamater(verticalarrows).NucleiarestainedwithDAPI(blue).
- 1.Bhat,H.F.etal.(2014)Br.J.Cancer.110,706.
- 2.Wilde,A.A.etal.(2011)Circ.Res.108,884.
- 3.Willis,B.C.etal.(2105)Am.J.Physiol.Heart.Circ.Physiol.308,1463.
- 4.Anderova,M.etal.(2014)PLoSOne9,e113444.
- 5.Williams,J.C.etal.(006)J.Biol.Chem.281,23341.
- 6.Koo,T.etal.(2011)Hum.Gene.Ther.22,1379.
α1-Syntrophinisacomponentofthedystrophinscaffold-proteincomplexthatactsinrecruitingproteinsthroughtheirmultipleprotein–proteininteractionmotifstothecytoplasmicsideofplasmamembranes.Proteinsinteractingwithα1-syntrophinincludevoltage-gatedsodiumchannels,neuronalnitricoxidesynthase,calmodulin,ankyrinrepeat-richmembranespanningprotein,G-proteinsandmore.
Thesyntrophinfamilycomprisesfivemembers:α1-syntrophin,β1-syntrophin,β2-syntrophin,γ1-syntrophin,andγ2-syntrophin1.
α1-Syntrophinisaperipheralcytoplasmicmembraneproteininskeletalandcardiacmusclecells1.
Theα1-syntrophinstructurecontainsfourconserveddomains,twopleckstrinhomologydomains(PH1andPH2),aPSD95-diskslarge-ZO1(PDZ)domainandasyntrophinunique(SU)COOH-terminaldomain2.ThepleckstrindomainsareresponsIBLefortherecruitmentofproteinstothesarcolemma.ThePDZdomainisinsertedwithinthePH1domainandbindstoNOS-1inskeletalmuscle.ThisdomainalsobindstotheC-terminusofthe Kir2.1 channelandalsoto NaV1.5 andsoallowingthemtointeract.TheSUdomainbindssyntrophintodystrophin2,3.α1-Syntrophinisalsoresponsibleforthesite-specificanchoringof AQP4 protein.α1-SyntrophindeletionleadstothedisruptionofthepolarizedsubcellularexpressionofAQP4intheperivascularmembranes4.
Severalstudieshaveshownthatsyntrophin-deficiencyinDuchennemusculardystrophycanultimatelyleadtomuscledegeneration.Syntrophin-deficiencywasalsoshowntoreducelevelsofnNOSinmice5,6.

AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanepitopeofmousesyntrophinalpha1.Anti-α1-Syntrophin(SNTA1)Antibody(#APZ-021)canbeusedinwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizesyntrophinalpha1frommouse,ratandhumansamples.
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