Overview
Product name
Human Cardiac Troponin I ELISA KitSee all Cardiac Troponin I kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Human heart 8 3% Inter-assay Sample n Mean SD CV% Human heart 3 7.1% Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
4.4 pg/mlRange
31.3 pg/ml -4000 pg/mlRecovery
Sample specific recovery Sample type Average % Range Serum 97 93%- 101% Tissue Extracts 112 104%- 118% Cell culture media 102 97%- 108% Hep Plasma 107 99%- 117% EDTA Plasma 93 90%- 97% Cit plasma 93 87%- 100% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:HumanProduct overview
As of November 7, 2018, Human Cardiac Troponin 1 ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.
Human Cardiac Troponin I ELISA Kit (ab200016) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cardiac Troponin I protein in serum, tissue extracts, cell culture extracts, hep plasma, cit plasma, edta plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cardiac Troponin I with 4.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Notes
The regulatory troponin complex regulates skeletal and cardiac muscle contraction. This complex, together with tropomyosin, is located on the actin filament and it is composed of three protein subunits: troponin T (the tropomyosin-binding subunit), troponin I (the inhibitory subunit, which inhibits the ATPase activity of acto-myosin), and troponin C (the Ca2+-binding subunit). Troponins T and I have unique cardiac isoforms, whereas cardiac and skeletal muscle share troponin C. Specifically, three human troponin I isoforms have been described: one is expressed in cardiac muscle (Cardiac Troponin I) and the other two are found in slow-twitch and fast-twitch skeletal muscle fibers (slow sTnI and fast sTnI, respectively). The sequence homology between Cardiac Troponin I and slow sTnI is approximately 40% and somewhat less for fast sTnI. Cardiac Troponin I is 209 amino acid long with a molecular weight of approximately 24 kDa. Mouse and rat Cardiac Troponin I proteins both show 93% amino acid identity to human Cardiac Troponin I. The presence of human Cardiac Troponin I in serum (together with chest pain and electrocardiographic changes) is now considered as one highly specific biochemical marker of myocardial injury, risk stratification of acute coronary syndrome and myocardial infarction. Mutations of Cardiac Troponin I are associated with hereditary cardiomyopathy. Specifically, defects in Cardiac Troponin I are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intra-familial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in Cardiac Troponin I also cause cardiomyopathy familial restrictive type 1 (RCM1). RCM1 is a heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. Furthermore, cardiomyopathy dilated type 2A (CMD2A) and cardiomyopathy dilated type 1FF (CMD1FF), disorders characterized by ventricular dilation and impaired systolic function resulting in congestive heart failure and arrhythmia, are caused by defects in Cardiac Troponin I.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Platform
Microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Human Cardiac Troponin I Capture Antibody 1 x 600µl 10X Human Cardiac Troponin I Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody DiluentCPI - HAMA Blocker (ab193969) 1 x 6ml Human Cardiac Troponin I Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Cellular localization
Cytosol; Sarcomere.Alternative names
- Cardiomyopathy dilated 2A (autosomal recessive)
- CMD1FF
- CMD2A
see allDatabase links
- Entrez Gene:7137 Human
- Omim:191044 Human
- SwissProt:P19429 Human
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Amsbio公司于 1987 年在西班牙马德里组建，在意大利，瑞士和英国开设了多处办事处。
AMSBIO是优质生命科学研究试剂和服务的主要供应商，帮助客户开发创新的方法、工艺、和产品。
AMSBIO’s mission is to be a profitable premier provider of quality life science research reagents and services helping customers develop innovative methods, processes, products and medicines. This is achieved by offering small and medium size manufacturers, academic groups and revenue generating biotechs a unique partnership for the global market and by providing state of the art and cost effective solutions to end users and partners.
产品列表：
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No. |
品牌 |
货号 |
名称 |
规格 |
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1 |
amsbio |
ASR-3053 |
MyEZGel iPSC 3D Cell Culture Matrix |
2ml |
|
2 |
amsbio |
11889 |
CELLBANKER 1 |
20ml |
|
3 |
amsbio |
11892 |
CELLBANKER 2 |
20ml |
|
4 |
amsbio |
11900 |
HSC-BANKER - GMP Grade |
15ml |
|
5 |
amsbio |
11897 |
STEM-CELLBANKER - GMP Grade |
20ml |
|
6 |
amsbio |
11897F |
STEM-CELLBANKER DMSO FREE - GMP Grade |
20ml |
|
7 |
amsbio |
TA50011-100 |
100ul |
|
|
8 |
amsbio |
TA150021 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, DyLight 488 |
100ul |
|
9 |
amsbio |
AU.0100.ALSI |
Aluminosilicate Glass Slides 10nm Gold (Au) over 2nm Ti Adhesion Layer - 1 inch x 3 inch x 0.7mm |
5 slides |
|
10 |
amsbio |
MDKT00010005 |
MagSi-NGSPREP Plus |
5ml |
|
11 |
amsbio |
MDKT00040008 |
MagSi-DT Removal |
8ml |
|
12 |
amsbio |
MD01016 |
MagSi-DNA 600 |
2ml |
|
13 |
amsbio |
MD01018 |
MagSi-DNA allround |
2ml |
|
14 |
amsbio |
MD01020 |
MagSi-DNA allround COOH |
2ml |
|
15 |
amsbio |
MD01024 |
MagSi-DNA 3.0 COOH |
2 mL |
苏州蚂蚁淘生物科技有限公司专注于为科研工作者提供CST，Abcam，Sigma，biorbyt，Abclonal，AbD Serotec，GeneTex，ENZO，ScienCell，BI，Swant，emfret，Biovision，Roche，Millipore，BD，Life，eBioscience，Thermo，Qiagen，Santa，Peprotech，R&D，ImmunoStar，GenStar，GeneCopoeia，Cusabio，Bioss，NeoBioscience
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Andrew Lees博士于2006年创立了Fina Biosolutions，以帮助新兴市场的疫苗制造商学习制造负担得起的疫苗蛋白多糖结合物。FinaBio现在是为全球生物技术和疫苗行业提供结合疫苗实验室和咨询服务的主要提供商。我们位于马里兰州生物技术走廊的中心，靠近美国国立卫生研究院（NIH），食品药品管理局（FDA）和许多全球生物制药公司。我们拥有一个设备完善的实验室，用于分子生物学
本人最近打算准备利用crisprcas9技术敲除老鼠细胞的基因，但是感觉问题好多呀，首先，我找到两条这个基因的CDNA序列，但是这两条序列不一样，只有89%的同源性，请问这应该怎么破？有没有大神可以指导一下啊？
sciventions的二氧化钛纳米粒子
TiO2纳米颗粒，由聚丙烯酸钠稳定。Sciventions二氧化钛
（-）纳米颗粒表现出出色的光催化作用，并且在很宽的pH范围内都稳定。它们是需要稳定，超小，水分散性纳米粒子的应用的理想选择。可以使用EDC（碳二亚胺）偶联化学方法将Scivention的TiO2（-）纳米颗粒与蛋白质或抗体偶联。
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最好是测浓度，一般不超过30-40ug，上样缓冲液一般4×或者5×，这个没太大区别。
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苏州蚂蚁淘生物科技有限公司是专业从事生物技术产品销售的高科技企业，是国内生物试剂、仪器、消耗品领域的知名企业之一。起发公司自成立以来，连续2年高速成长。公司的发展目标是建成专业从事生物技术产品销售、生产与开发的一流公司。
