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BD Biosciences/HLA-B27 Kit/340183

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Bacteria

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340183

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4000-520-616

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Product DetailsRecommended AssayReferences

Description

The BD™ HLA-B27 system is a qualitative two-color direct immunoﬂuorescence method for the rapid detection of HLA-B27 antigen expression in erythrocyte-lysed whole blood (LWB) using the BD FACSCanto™ family of ﬂow cytometers, or the BD FACSCalibur™, BD FACSort™, or BD FACScan™ ﬂow cytometer.

Contents: Anti-HLA-B27 FITC / CD3 PE, HLA-B27 calibration beads, and BD FACS™ Lysing Solution.

Resources & Tools
SpectrumViewer	CFDA License	Download TDS	Download MSDS

Preparation and Storage

• Store Reagent A and Reagent C upright at 2° to 8°C. Do not use after the expiration date shown on the label.

• Store Reagent B, undiluted, at 2° to 25°C. When diluted, store at room temperature (20° to 25°C) for up to one month.

• Do not freeze the reagents or expose them to direct light during storage or incubation with cells. Keep reagent vials dry.

Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture–Fourth Edition; Approved Standard. Wayne, PA: National Committee for Clinical Laboratory Standards; 1998. NCCLS document H3-A4.
Protection of Laboratory Workers from Occupationally Acquired Infections–Second Edition; Approved Guideline. Villanova, PA: National Committee for Clinical Laboratory Standards; 2001. NCCLS document M29-A2.
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.
Dausset J. The major histocompatibility in man. Past, present, and future concepts. Science. 1981;213:1469-1474.
Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. New York, NY: Springer-Verlag; 1986;1:3-30.
Johnson J, Garbutt J, Nelson K. A monoclonal antibody speciﬁc for HLA-B27. Human Immunol. 1985;134-135.
Kan EAR, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti–Leu-4 antibody. J Immunol. 1983;131:536-539.
Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. New York, NY: Springer-Verlag; 1986;1:259-288.
Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T-lymphocyte helper/inducer and T cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981;153:310-323.
Levering WH, Wind H, Sintnicolaas K, Hooijkaas H, Gratama JW. Flow cytometric HLA-B27 screening: cross-reactivity patterns of commercially available anti-HLA-B27 monoclonal antibodies with other HLA-B antigens. Cytometry B Clin Cytom. 2003;54B:28-38.
Lopez-Larrea C, Gonzalez-Roces S, Alvarez V. HLA-B27 structure, function, and disease association. Curr Opin Rheumatol. 1996;8:296-308.
Müller H, Albrecht J, Schabel A. New aspects in HLA-typing by use of monoclonal antibodies and calibrated ﬂow cytometry. Fresenius A Anal Chem. 1988;330:341.
Seipp MT, Erali M, Wies RL, Wittwer C. HLA-B27 typing: evaluation of an allele-speciﬁc PCR melting assay and two ﬂow cytometric antigen assays. Cytometry B Clin Cytom. 2005;63:10-15.
Taurog JD, El-Zaatari FA. In vitro mutagenesis of HLA-B27. Substitution of an unpaired cysteine residue in the alpha 1 domain causes loss of antibody-deﬁned epitopes. J Clin Invest. 1988;82:987-992.

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蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁淘搜索全球供应信息，找到合适的产品后在蚂蚁淘下单，然后蚂蚁淘的海外买手进行跨境采购、运输到中国口岸，最后由蚂蚁淘国内团队报关运输给客户...

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