Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
dT-Me Phosphoramidite
dT-Me Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1330-02 | dT-Me Phosphoramidite | 0.25g | £30.00£28.50Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1330-05 | dT-Me Phosphoramidite | 0.5g | £40.00£38.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1330-10 | dT-Me Phosphoramidite | 1.0g | £80.00£76.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
dT-Me Phosphoramidite
dT-Me Phosphoramidite
Glen Research
| dT-Me Phosphoramidite |
Catalog Number: 10-1330-xx
Description: dT-Me Phosphoramidite
| 5"-Dimethoxytrityl-2"-deoxyThymidine,3"-[(methyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C38H48N3O8P | M.W.: 705.79 | F.W.: 318.22 (Methyl triester) |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature to leave the methyl phosphotriester intact.Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
METHYL PHOSPHORAMIDITES
For many years, Glen Research has supplied methyl phosphoramidites in addition to þ-cyanoethyl (CE) phosphoramidites for the few situations where the more labile cyanoethyl group is not an advantage. Some of our customers, probably remembering that the methyl group was removed specifically with thiophenol, have tried to use these monomers to prepare the interesting, uncharged, and nuclease-resistant methyl phosphotriester linkage. Unfortunately, this linkage is labile to ammonium hydroxide and the regular phosphodiester linkage is formed (along with a small amount of chain scission). Now we offer UltraMild methyl phosphoramidites for this application. Oligos produced from these monomers can be deprotected with potassium carbonate in methanol to produce methyl phosphotriester linkages. Since these linkages are diastereomeric and uncharged, the oligos may be hard to handle. Consequently, it is likely that chimeras will be produced using these monomers along with the regular UltraMild CE phosphoramidites. If many dG residues are included in the oligonucleotide, we recommend the use of phenoxyacetic anhydride (PAc2O) in Cap A. This modification removes the possibility of exchange of the isopropyl-phenoxyacetate (iPr-Pac) protecting group on the dG with acetate from the acetic anhydride capping mix.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
dT-Me Phosphoramidite
dT-Me Phosphoramidite
Glen Research
Material Safety Data Sheet
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
dT-Me Phosphoramidite
dT-Me Phosphoramidite
Glen Research
REQUENTLY ASKED TECHNICAL QUESTION
QUESTION: Can Me phosphoramidites (10-1300, etc.) be used to produce methyl triester oligonucleotides?
RESPONSE:Possibly, but we are unaware of a method. Some people remember that the methyl phosphoramidites were used to prepare oligos originally and that thiophenol was used to remove the methyl group prior to ammonium hydroxide deprotection. This might suggest that omitting the thiophenol step would lead to the methyl triesters. Wrong. Thiophenol was used to remove the methyl group specifically prior to the ammonium hydroxide step to avoid chain scission. If the methyl triesters are treated with ammonium hydroxide, they are predominantly hydrolyzed to the phosphodiesters but a small percentage of the linkages are also hydrolyzed to eliminate either the 3"or 5" alcohol (chain scission).
While working with dT-Me Phosphoramidite and deprotecting under UltraMild conditions, it was clear that the methyl triester group was essentially unreacted over 24 hours in potassium carbonate in methanol. We have now introduced UltraMild Me Phosphoramidite monomers and, finally, methyl triester oligonucleotides can be simply produced.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
dT-Me Phosphoramidite
dT-Me Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1330-02 | 0.25grams | .25grams | 3.54 | 104.67 | 62.8 | 39.25 | 28.55 | 20.93 | 5.23 |
| 10-1330-05 | 0.5grams | .5grams | 7.08 | 222.67 | 133.6 | 83.5 | 60.73 | 44.53 | 11.13 |
| 10-1330-10 | 1.0gram | 1grams | 14.17 | 459 | 275.4 | 172.13 | 125.18 | 91.8 | 22.95 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1330-02 | 0.25grams | .25grams | 5.29 | .07 | 99.4 | 62.13 | 45.18 | 6.21 | |
| 10-1330-05 | 0.5grams | .5grams | 10.57 | .07 | 205 | 128.13 | 93.18 | 12.81 | |
| 10-1330-10 | 1.0gram | 1grams | 21.15 | .07 | 416.6 | 260.38 | 189.36 | 26.04 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1330-02 | 0.25grams | .25grams | 5.29 | .07 | 101 | 63.13 | 45.91 | ||
| 10-1330-05 | 0.5grams | .5grams | 10.57 | .07 | 206.6 | 129.13 | 93.91 | ||
| 10-1330-10 | 1.0gram | 1grams | 21.15 | .07 | 418.2 | 261.38 | 190.09 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
AthensResearch&Technology公司位于美国乔治亚州雅典市，从1986年以来一直致力于纯化高纯度，高活性的人类蛋白质和开发这些蛋白的多克隆抗血清。我们的常规产品包括丝氨酸蛋白酶、蛋白酶抑制剂、中性粒细胞酶、载脂蛋白、脂蛋白，血小板蛋白、转铁蛋白、免疫球蛋白等。二十年多年来，世界各地的研究人员都在使用我们公司的产品。在炎症、冠状动脉疾病、自身免疫性疾病、癌症、老年痴呆症等等研究的主要科学出版物中都能看到引用我们公司的产品。我们提供优质，可靠的产品和出色的客户服务。我们期待着您的业务。如果需要定制研究服务，AthensResearch&Technology公司能够根据您的项目要求提供无与伦比的试剂。
公司主要产品：一、凝血因子相关系列产品：包括多款从人血浆、血小板中分离纯化的凝血因子相关蛋白。二、酶及抑制剂系列产品：包括多款从人血浆、红细胞分离纯化的a-抗糜蛋白酶、a1/2抗胰蛋白酶、抗凝血酶、C1酯酶抑制因子、Calpain-1等酶及其抑制剂产品。三、免疫球蛋白系列产品：包括多款从人血浆、骨髓瘤、初乳以及狗和小鼠血浆中分离纯化的免疫球蛋白产品。四、脂蛋白及载脂蛋白系列产品：包括包括多款从人血浆中分离纯化的载脂蛋白及脂蛋白（高密度、中等密度、低密度、极低密度脂蛋白）产品。五、低内毒素蛋白系列产品：包括多款适宜于细胞培养的低内毒素脂蛋白、免疫球蛋白及转铁蛋白产品。六、乳蛋白系列产品：包括从人乳中分离纯化的乳白蛋白、乳铁传递蛋白及分泌型免疫球蛋白A。七、中性粒细胞源蛋白系列产品：包括多款纯化分离自人中性粒细胞的Azurocidin 、CathepsinG及Lactoferrin等蛋白产品。八、血小板相关系列产品：包括纯化分离自人血小板的血小板因子4及凝血酶致敏蛋白。九、转铁蛋白及脱铁转铁蛋白（APOTRANSFERRINS）系列产品：包括多款纯化分离自人、大小鼠、狗及食蟹猴血浆中的转铁蛋白/脱铁转铁蛋白，可应用于细胞或组织培养。十、抗体系列产品：包括多款兔、大鼠、羊源的多克隆抗体产品。
ebiomall.com
