Oligo Synthesis : CEPs
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EDTA-C2-dT-CE Phosphoramidite
EDTA-C2-dT-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1059-02 | EDTA-C2-dT-CE Phosphoramidite | 0.25g | £780.00£741.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1059-90 | EDTA-C2-dT-CE Phosphoramidite | 100µmoles | £396.00£376.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1059-95 | EDTA-C2-dT-CE Phosphoramidite | 50µmoles | £200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
EDTA-C2-dT-CE Phosphoramidite
EDTA-C2-dT-CE Phosphoramidite
Glen Research
| EDTA-C2-dT-CE Phosphoramidite |
Catalog Number: 10-1059-xx
Description: EDTA-C2-dT-CE Phosphoramidite
| 5"-Dimethoxytrityl-5-[N-ethylenediaminetriethylacetate,monoacetylaminoethyl-3-acrylimido]-2"-deoxyUridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C60H81N8O16P | M.W.: 1201.32 | F.W.: 676.53 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 6 minute coupling time recommended. |
| Deprotection: 0.4M methanolic sodium hydroxide (methanol:water 4:1) at room temperature for 17 hours.Note: NaOH is not compatible with dmf protecting groups. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
Stability in Solution: 2-3 days EDTA LABELLINGEDTA-C2-dT phosphoramidite contains the triethyl ester of EDTA which allows sequence-specific cleavage of single- and double-stranded DNA and RNA. The cleavage reaction is only initiated once Fe(II) and dithiothreitol are added and so is readily controlled. Coupling of EDTA-dT is normal but cleavage and deprotection should be carried out with sodium hydroxide in aqueous methanol (0.4M NaOH in methanol/water 4:1) overnight at room temperature. |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
EDTA-C2-dT-CE Phosphoramidite
EDTA-C2-dT-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
| Glen Report 11.2: MORE NOVEL MONOMERS |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
EDTA-C2-dT-CE Phosphoramidite
EDTA-C2-dT-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1059-02 | 0.25grams | .25grams | 2.08 | 56 | 33.6 | 21 | 15.27 | 11.2 | 2.8 |
| 10-1059-90 | 100µmoles | .12grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1059-95 | 50µmoles | .06grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1059-02 | 0.25grams | .25grams | 3.11 | .07 | 55.8 | 34.88 | 25.36 | 3.49 | |
| 10-1059-90 | 100µmoles | .12grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1059-95 | 50µmoles | .06grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1059-02 | 0.25grams | .25grams | 3.11 | .07 | 57.4 | 35.88 | 26.09 | ||
| 10-1059-90 | 100µmoles | .12grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1059-95 | 50µmoles | .06grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
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