Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
Spermine Phosphoramidite
Spermine Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1939-02 | Spermine Phosphoramidite | 0.25g | £430.00£399.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1939-90 | Spermine Phosphoramidite | 100 µmole | £216.00£205.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1939-95 | Spermine Phosphoramidite | 50 µmole | £116.00£110.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Spermine Phosphoramidite
Spermine Phosphoramidite
Glen Research
Spermine Phosphoramidite
Catalog Number: 10-1939-xx
Description: Spermine Phosphoramidite
| N1-[4-(4,4"-Dimethoxytrityloxy)butyl]-N1,N4,N9,N12-tetrakis(trifluoroacetyl)-spermine-N12-butyl-4-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C56H73F12N6O9P | M.W.: 1233.17 | F.W.: 408.52 |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/ProductFiles/Technical/TB_ABITransfer.pdf. |
| Coupling: 3 minute coupling time recommended. |
| Deprotection: Prior to cleavage, treat support with 10% diethylamine in acetonitrile for 5 minutes at room temperature and rinse with acetonitrile to remove the cyanoethyl protecting groups. Then deprotect using 30% ammonium hydroxide as required by nucleobases. (Note a minimum of 4 hours at room temperature, or approximately 30 minutes at 55°C, is required to remove the TFA protecting groups.) |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
| Please Note: "Spermine phosphoramiditeÓ synthon is the subject matter of U.S. Patent Application No. 12/086.599, European Patent Application No. EP20060847298 and foreign equivalents for which Polyplus-transfection is the co-owner. Product is sold for research purposes only. Product shall not be used to manufacture oligospermine-oligonucleotide conjugates for use in diagnostics, clinical or commercial applications including use in humans. There is no implied license to manufacture oligospermine-oligonucleotide conjugates for diagnostic, clinical, or commercial applications, including but not limited to contract research. Please contact Polyplus-transfection at licensing@polyplus-transfection.com to obtain a license for such use. |
ZIP NUCLEIC ACIDS (ZNA®)
Spermine phosphoramidite is used to produce oligospermine-oligonucleotide conjugates - Zip Nucleic Acids (ZNA®) Oligos. The name reflects the presumed mode of action. The conjugates are believed to use the oligospermine to seek out and move along (scan) oligonucleotide strands until the probe complementary sequence is located. The oligospermine then performs the function of stabilizing the formed duplex by reducing electrostatic repulsion, thereby leading to significantly increased binding affinities. ZNA® Oligos have found use in the following applications: Multiplex PCR; PCR of AT-rich Regions; RT qPCR; Detection of MicroRNA; Improved SNP Discrimination; and Antisense and Antigene Effects. Spermine phosphoramidite is simple to use in oligonucleotide synthesis and can be added multiple times at the 3’ or 5’ terminus. Deprotection and isolation are also straightforward. HPLC analysis of the conjugates requires high pH to suppress the ionization of the spermine residues.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Spermine Phosphoramidite
Spermine Phosphoramidite
Glen Research
Material Safety Data Sheet
| Glen Report 24.1: Spermine Phosphoramidite: A potent modification with many applications |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Spermine Phosphoramidite
Spermine Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1939-95 | 50µmoles | .062grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| 10-1939-90 | 100µmoles | .123grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1939-02 | 0.25grams | .25grams | 2.03 | 54.33 | 32.6 | 20.38 | 14.82 | 10.87 | 2.72 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1939-95 | 50µmoles | .062grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| 10-1939-90 | 100µmoles | .123grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1939-02 | 0.25grams | .25grams | 3.03 | .07 | 54.2 | 33.88 | 24.64 | 3.39 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1939-95 | 50µmoles | .062grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
| 10-1939-90 | 100µmoles | .123grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1939-02 | 0.25grams | .25grams | 3.03 | .07 | 55.8 | 34.88 | 25.36 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
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