Oligo Synthesis : CEPs
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U-CE Phosphoramidite
U-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-3030-02 | U-CE Phosphoramidite | 0.25g | £32.00£30.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3030-05 | U-CE Phosphoramidite | 0.5g | £64.00£60.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3030-10 | U-CE Phosphoramidite | 1.0g | £128.00£121.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
U-CE Phosphoramidite
U-CE Phosphoramidite
Glen Research
| U-CE Phosphoramidite |
Catalog Number: 10-3030-xx
Description: U-CE Phosphoramidite
| 5"-Dimethoxytrityl-Uridine,2"-O-TBDMS,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C45H61N4O9PSi | M.W.: 861.06 | F.W.: 306.17 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 12 minute coupling time recommended |
| Deprotection: RNA Deprotection-see bulletinTechnical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 24 hours |
TOM-PROTECTED RNA PHOSPHORAMIDITES
RNA synthesis using monomers containing the 2’-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection. High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2’-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers. Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature. A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2’ to 3’ migration. This migration under basic conditions leads to non-biologically active 2’-5’ linkages when using the TBDMS group. These features allow the TOM-Protected monomers to produce longer oligonucleotides. TOM-Protected RNA monomers are also fully compatible with minor bases with 2’-O-TBDMS protection.
TBDMS-PROTECTED RNA PHOSPHORAMIDITES
Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certificate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling efficiency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in use. Dmf-G (10-3029) has been discontinued and may be substituted with Ac-G (10-3025).
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
U-CE Phosphoramidite
U-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 9.1: PROTECTING GROUPS FOR DNA, RNA AND 2"-OMe-RNA MONOMERS
Glen Report 19.2: New Lines of Monomers - HT for High Throughput and LC for low cost RN
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
U-CE Phosphoramidite
U-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3030-02 | 0.25grams | .25grams | 2.9 | 83.33 | 50 | 31.25 | 22.73 | 16.67 | 4.17 |
| 10-3030-05 | 0.5grams | .5grams | 5.81 | 180.33 | 108.2 | 67.63 | 49.18 | 36.07 | 9.02 |
| 10-3030-10 | 1.0gram | 1grams | 11.61 | 373.67 | 224.2 | 140.13 | 101.91 | 74.73 | 18.68 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3030-02 | 0.25grams | .25grams | 4.33 | .07 | N/A | N/A | 16.71 | N/A | |
| 10-3030-05 | 0.5grams | .5grams | 8.67 | .07 | N/A | N/A | 34.79 | N/A | |
| 10-3030-10 | 1.0gram | 1grams | 17.33 | .07 | N/A | N/A | 70.88 | N/A | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3030-02 | 0.25grams | .25grams | 4.33 | .07 | N/A | 13.19 | 12.39 | ||
| 10-3030-05 | 0.5grams | .5grams | 8.67 | .07 | N/A | 27.19 | 25.55 | ||
| 10-3030-10 | 1.0gram | 1grams | 17.33 | .07 | N/A | 55.13 | 51.79 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
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