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Athens Research/Cambio - Excellence in Molecular Biology/1.5 ml/EZT-22RV-1-15
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Athens Research/Cambio - Excellence in Molecular Biology/1.5 ml/EZT-22RV-1-15
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Transfection - EZ Biosystems

Transfection - EZ Biosystems: Cell Lines

Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.

For primary cells click HERE

To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE

  • Catalogue
  • Description

22Rv1 Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell)

22Rv1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 22Rv1 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 22Rv1 cells.

EZ Biosystems

Catalogue No.DescriptionPack SizePriceQty
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  • €
EZT-22RV-1-0522Rv1 Cell Transfection Reagent (human prostate carcinoma cell)0.5 ml£399.00QuantityAdd to Order
EZT-22RV-1-1522Rv1 Cell Transfection Reagent (human prostate carcinoma cell)1.5 ml£699.00QuantityAdd to Order

22Rv1 Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell)

22Rv1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 22Rv1 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 22Rv1 cells.

EZ Biosystems

Cell LineInformation:

Designations: 22Rv1 Organism: Homo sapiens (human) Tissue: carcinoma; prostate Morphology: epithelial Growth properties: adherent Tumorigenic: yes, forms tumors in nude mice Antigen expression: prostate specific antigen (PSA) DoublingTime: 40 hours Receptors expressed: androgen Depositors: J.W. Jacobberger 22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft. The cell line expresses prostate specific antigen. Growth is stimulated by epidermal growth factor (EGF) but is not inhibited by transforming growth factor beta-1 (TGF beta- 1).

The Transfection Reagent:

22Rv1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 22Rv1 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 22Rv1 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

  • Unique formulation-maximize transfection performance in 22Rv1 cells.
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • Deliver single or multiple plasmids
  • Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on PC-3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for PC-3 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on 22Rv1 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as 22Rv1 Cell Avalanche Transfection Reagent.
FIG. 2. 22Rv1 cells were transfected with GFP vector (pEGFP-N3) by using 22Rv1 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells:
22Rv1 Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell) can also be used on the following cells with high transfection efficiencies.

DU 145 Cell VCaP Cell PC-3 Cell Vero Cell 293 Cell 293T/17 Cell Calu-3 Cell Caki-1 Cell MDCK Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

AthensResearch&Technology公司位于美国乔治亚州雅典市,从1986年以来一直致力于纯化高纯度,高活性的人类蛋白质和开发这些蛋白的多克隆抗血清。我们的常规产品包括丝氨酸蛋白酶、蛋白酶抑制剂、中性粒细胞酶、载脂蛋白、脂蛋白,血小板蛋白、转铁蛋白、免疫球蛋白等。二十年多年来,世界各地的研究人员都在使用我们公司的产品。在炎症、冠状动脉疾病、自身免疫性疾病、癌症、老年痴呆症等等研究的主要科学出版物中都能看到引用我们公司的产品。我们提供优质,可靠的产品和出色的客户服务。我们期待着您的业务。如果需要定制研究服务,AthensResearch&Technology公司能够根据您的项目要求提供无与伦比的试剂。


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