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ProteinTech/SOD1 Polyclonal antibody/150ul/10269-1-AP

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ProteinTech/SOD1 Polyclonal antibody/150ul/10269-1-AP

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ProteinTech

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10269-1-AP

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Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells,mouse liver tissue,Jurkat cells,human placenta tissue,mouse brain tissue,rat brain tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human liver cancer tissue, human lung tissue,human placenta tissue,human testis tissue,human skin tissue,human heart tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 80 publications below
IHC	See 9 publications below
IF	See 5 publications below
IP	See 1 publications below

Product Information

10269-1-AP targets SOD1 inWB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	broiler chick, human, mouse, rat, swine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SOD1 fusion protein Ag0335
Full Name	superoxide dismutase 1, soluble
Calculated molecular weight	16 kDa
Observed molecular weight	16-20 kDa
GenBank accession number	BC001034
Gene symbol	SOD1
Gene ID (NCBI)	6647
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.

Protocols

Product Specific Protocols
WB protocol for SOD1 antibody 10269-1-AP	Download protocol
IHC protocol for SOD1 antibody 10269-1-AP	Download protocol
IF protocol for SOD1 antibody 10269-1-AP	Download protocol
IP protocol for SOD1 antibody 10269-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

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