Tested Applications
| Positive WB detected in | Staurosporine treated Jurkat cells, mouse brain tissue,Jurkat cells |
| Positive IHC detected in | human prostate cancer tissue, human lung cancer tissue,human skin tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
Product Information
10198-1-AP targets Caspase 6/p18/p11 inWB, IHC, ELISA applications and shows reactivity with human,mouse,rat samples.
| Tested Reactivity | human,mouse,rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Caspase 6/p18/p11 fusion protein Ag0257 |
| Full Name | caspase 6, apoptosis-related cysteine peptidase |
| Calculated molecular weight | 33 kDa, 22 kDa |
| Observed molecular weight | 33-35 kDa |
| GenBank accession number | BC000305 |
| Gene symbol | CASP6 |
| Gene ID (NCBI) | 839 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 6/p18/p11 antibody 10198-1-AP | Download protocol |
| IHC protocol for Caspase 6/p18/p11 antibody 10198-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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