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ProteinTech/Bcl-XL Polyclonal antibody/150ul/10783-1-AP

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ProteinTech/Bcl-XL Polyclonal antibody/150ul/10783-1-AP

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ProteinTech

货号 /

10783-1-AP

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Tested Applications

Positive WB detected in	A549 cells, Raji cells,mouse placenta tissue,HepG2 cells,C6 cells,Jurkat cells,K-562 cells,human placenta tissue,NIH/3T3 cells,RAW264.7,HeLa cells,rat brain tissue
Positive IP detected in	K-562 cells
Positive IHC detected in	human lung cancer tissue,human colon tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in	NIH/3T3 cells
Positive FC detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 88 publications below
IHC	See 5 publications below

Product Information

10783-1-AP targets Bcl-XL inWB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human,mouse,rat samples.

Tested Reactivity	human,mouse,rat
Cited Reactivity	goat, human, mouse, rat, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Bcl-XL fusion protein Ag1232
Full Name	BCL2-like 1
Calculated molecular weight	26 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC019307
Gene symbol	BCL2L1
Gene ID (NCBI)	598
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta) , and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein. This antibody can recognize Bcl-XL, Bcl-X(s) and Bcl-X(Beta).

Protocols

Product Specific Protocols
WB protocol for Bcl-XL antibody 10783-1-AP	Download protocol
IHC protocol for Bcl-XL antibody 10783-1-AP	Download protocol
IF protocol for Bcl-XL antibody 10783-1-AP	Download protocol
IP protocol for Bcl-XL antibody 10783-1-AP	Download protocol
FC protocol for Bcl-XL antibody 10783-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

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