Tested Applications
| Positive WB detected in | K-562 cells,HEK-293 cells,HeLa cells,MCF-7 cells,mouse heart tissue,mouse kidney tissue,mouse liver tissue,mouse skeletal muscle tissue,mouse testis tissue,Raji cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human colon cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
10401-1-AP targets RAIDD inWB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RAIDD fusion protein Ag0630 |
| Full Name | CASP2 and RIPK1 domain containing adaptor with death domain |
| Calculated molecular weight | 23 kDa |
| Observed molecular weight | 23 kDa |
| GenBank accession number | BC017042 |
| Gene symbol | CRADD |
| Gene ID (NCBI) | 8738 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
RAIDD, also named as CRADD, is an apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. CRADD interacts with BCL10 through its caspase recruitment domain and suppresses interactions between BCL10 and CARMA1. It is a negative regulator of the CARMA1 signalosome and suppressor of Th1-and Th17-mediated inflammatory responses.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAIDD antibody 10401-1-AP | Download protocol |
| IHC protocol for RAIDD antibody 10401-1-AP | Download protocol |
| IP protocol for RAIDD antibody 10401-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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