
Tested Applications
Positive WB detected in | K-562 cells,HEK-293 cells,HeLa cells,MCF-7 cells,mouse heart tissue,mouse kidney tissue,mouse liver tissue,mouse skeletal muscle tissue,mouse testis tissue,Raji cells |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human colon cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
Product Information
10401-1-AP targets RAIDD inWB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RAIDD fusion protein Ag0630 |
Full Name | CASP2 and RIPK1 domain containing adaptor with death domain |
Calculated molecular weight | 23 kDa |
Observed molecular weight | 23 kDa |
GenBank accession number | BC017042 |
Gene symbol | CRADD |
Gene ID (NCBI) | 8738 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
RAIDD, also named as CRADD, is an apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. CRADD interacts with BCL10 through its caspase recruitment domain and suppresses interactions between BCL10 and CARMA1. It is a negative regulator of the CARMA1 signalosome and suppressor of Th1-and Th17-mediated inflammatory responses.
Protocols
Product Specific Protocols | |
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WB protocol for RAIDD antibody 10401-1-AP | Download protocol |
IHC protocol for RAIDD antibody 10401-1-AP | Download protocol |
IP protocol for RAIDD antibody 10401-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
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