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ProteinTech/ERK1/2 Polyclonal antibody/150ul/16443-1-AP

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ProteinTech/ERK1/2 Polyclonal antibody/150ul/16443-1-AP

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ProteinTech

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16443-1-AP

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4000-520-616

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Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue,NIH/3T3 cells,A431 cells,mouse kidney tissue,HEK-293 cells,PC-3 cells,rat brain tissue,rat kidney tissue
Positive IHC detected in	human liver tissue, human kidney tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 169 publications below
IHC	See 2 publications below
IF	See 2 publications below

Product Information

16443-1-AP targets ERK1/2 inWB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	A. sphaerocephala Krasch, Astragalus membranaceus, chicks, human, mouse, pig, rat, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ERK1/2 fusion protein Ag9772
Full Name	mitogen-activated protein kinase 1
Calculated molecular weight	360 aa, 41 kDa
Observed molecular weight	38-44 kDa
GenBank accession number	BC017832
Gene symbol	MAPK1
Gene ID (NCBI)	5594
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Protocols

Product Specific Protocols
WB protocol for ERK1/2 antibody 16443-1-AP	Download protocol
IHC protocol for ERK1/2 antibody 16443-1-AP	Download protocol
IF protocol for ERK1/2 antibody 16443-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

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