
Tested Applications
Positive WB detected in | NIH/3T3 cells, K-562 cells,MCF-7 cells,HepG2 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human kidney tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 4 publications below |
Product Information
The immunogen of 23762-1-AP is RSK2 Fusion Protein expressed in E. coli.
Tested Reactivity | human,mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RSK2 fusion protein Ag20620 |
Full Name | ribosomal protein S6 kinase, 90kDa, polypeptide 3 |
Calculated molecular weight | 740 aa, 84 kDa |
Observed molecular weight | 84 kDa |
GenBank accession number | BC096301 |
Gene symbol | RPS6KA3 |
Gene ID (NCBI) | 6197 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
RPS6KA3(Ribosomal protein S6 kinase alpha-3) is also named as ISPK1, MAPKAPK1B, RSK2 and belongs to the protein kinase superfamily. It localizes at the kinetochores under spindle checkpoint conditions, and during mitosis, associated with the centrosomes, the spindle and at the mid-body(PMID:20383198). It is required for EGF activated phosphorylation of histone H3, which may cause chromatin remodeling and facilitate gene transcription and maybe playing an essential regulatory role in protein synthesis during cell proliferation.
Protocols
Product Specific Protocols | |
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WB protocol for RSK2 antibody 23762-1-AP | Download protocol |
IHC protocol for RSK2 antibody 23762-1-AP | Download protocol |
IF protocol for RSK2 antibody 23762-1-AP | Download protocol |
IP protocol for RSK2 antibody 23762-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
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