
Tested Applications
Positive WB detected in | HEK-293 cells,Jurkat cells,mouse kidney tissue |
Positive IHC detected in | human liver cancer tissue,human lung cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 24777-1-AP is Caspase 2/p18 Fusion Protein expressed in E. coli.
Tested Reactivity | human,mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Caspase 2/p18 fusion protein Ag20141 |
Full Name | caspase 2, apoptosis-related cysteine peptidase |
Calculated molecular weight | 452 aa, 51 kDa |
Observed molecular weight | 45 kDa, 32 kDa |
GenBank accession number | BC002427 |
Gene symbol | CASP2 |
Gene ID (NCBI) | 835 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.Itis involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).
Protocols
Product Specific Protocols | |
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WB protocol for Caspase 2/p18 antibody 24777-1-AP | Download protocol |
IHC protocol for Caspase 2/p18 antibody 24777-1-AP | Download protocol |
IF protocol for Caspase 2/p18 antibody 24777-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
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