
Tested Applications
Positive WB detected in | HEK-293 cells, K-562 cells,MCF-7 cells,NIH/3T3 cells,HeLa cells,HL-60 cells,Jurkat cells,A431 cells,rat kidney tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human liver cancer tissue, human prostate cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 98 publications below |
IHC | See 4 publications below |
CoIP | See 1 publications below |
Product Information
10122-1-AP targets CDK2 inWB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human,mouse,rat samples.
Tested Reactivity | human,mouse,rat |
Cited Reactivity | human, mouse, rat, sea cucumbers, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CDK2 fusion protein Ag0166 |
Full Name | cyclin-dependent kinase 2 |
Calculated molecular weight | 33 kDa |
Observed molecular weight | 33 kDa |
GenBank accession number | BC003065 |
Gene symbol | CDK2 |
Gene ID (NCBI) | 1017 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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WB protocol for CDK2 antibody 10122-1-AP | Download protocol |
IHC protocol for CDK2 antibody 10122-1-AP | Download protocol |
IF protocol for CDK2 antibody 10122-1-AP | Download protocol |
IP protocol for CDK2 antibody 10122-1-AP | Download protocol |
FC protocol for CDK2 antibody 10122-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
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