Tested Applications
| Positive WB detected in | HEK-293 cells, K-562 cells,MCF-7 cells,NIH/3T3 cells,HeLa cells,HL-60 cells,Jurkat cells,A431 cells,rat kidney tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human prostate cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 98 publications below |
| IHC | See 4 publications below |
| CoIP | See 1 publications below |
Product Information
10122-1-AP targets CDK2 inWB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human,mouse,rat samples.
| Tested Reactivity | human,mouse,rat |
| Cited Reactivity | human, mouse, rat, sea cucumbers, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CDK2 fusion protein Ag0166 |
| Full Name | cyclin-dependent kinase 2 |
| Calculated molecular weight | 33 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | BC003065 |
| Gene symbol | CDK2 |
| Gene ID (NCBI) | 1017 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK2 antibody 10122-1-AP | Download protocol |
| IHC protocol for CDK2 antibody 10122-1-AP | Download protocol |
| IF protocol for CDK2 antibody 10122-1-AP | Download protocol |
| IP protocol for CDK2 antibody 10122-1-AP | Download protocol |
| FC protocol for CDK2 antibody 10122-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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