
Tested Applications
Positive WB detected in | K-562 cells |
Positive IHC detected in | human lymphoma tissue,human cervical cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 5 publications below |
IHC | See 1 publications below |
Product Information
60312-1-Ig targets CDK2 inWB, IHC, FC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgM |
Class | Monoclonal |
Type | Antibody |
Immunogen | CDK2 fusion protein Ag21310 |
Full Name | cyclin-dependent kinase 2 |
Calculated molecular weight | 33 kDa |
Observed molecular weight | 37 kDa |
GenBank accession number | BC003065 |
Gene symbol | CDK2 |
Gene ID (NCBI) | 1017 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Caprylic acid/ammonium sulfate precipitation |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage. |
Background Information
CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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WB protocol for CDK2 antibody 60312-1-Ig | Download protocol |
IHC protocol for CDK2 antibody 60312-1-Ig | Download protocol |
FC protocol for CDK2 antibody 60312-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
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