Tested Applications
| Positive WB detected in | mouse brain tissue, MCF-7 cells,Jurkat cells,rat brain tissue |
| Positive IHC detected in | human breast cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
Product Information
55103-1-AP targets CDK3 inWB, IHC, ELISA applications and shows reactivity with human,mouse samples.
| Tested Reactivity | human,mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | cyclin-dependent kinase 3 |
| Calculated molecular weight | 35 kDa |
| Observed molecular weight | 40-45 kDa |
| GenBank accession number | NM_001258 |
| Gene symbol | CDK3 |
| Gene ID (NCBI) | 1018 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for-20oC storage. |
Background Information
CDK3 belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It is probably involved in the control of the cell cycle. CDK3 interacts with a yet unknown type of cyclin. It can phosphorylate histone H1. Endogenous Cyclin C/Cdk3 complexes are critical to promote exit from quiescence in an Rb-dependent manner. Cyclin C/Cdk3 complexes is a key regulators of cell cycle reentry in human cells. (PMID: 15130482 )The antibody is specific to CDK3.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK3 antibody 55103-1-AP | Download protocol |
| IHC protocol for CDK3 antibody 55103-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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