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ProteinTech/CDK4 Polyclonal antibody/150ul/11026-1-AP

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ProteinTech/CDK4 Polyclonal antibody/150ul/11026-1-AP

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ProteinTech

货号 /

11026-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells,rat pancreas tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue, human breast cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)	IHC : 1:150-1:600
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 130 publications below
IHC	See 5 publications below
IF	See 1 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

11026-1-AP targets CDK4 inWB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	canine, human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CDK4 fusion protein Ag1505
Full Name	cyclin-dependent kinase 4
Calculated molecular weight	34 kDa
Observed molecular weight	34 kDa
GenBank accession number	BC010153
Gene symbol	CDK4
Gene ID (NCBI)	1019
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level(PMID:17253961). CCND1-CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth(PMID:21844184).

Protocols

Product Specific Protocols
WB protocol for CDK4 antibody 11026-1-AP	Download protocol
IHC protocol for CDK4 antibody 11026-1-AP	Download protocol
IP protocol for CDK4 antibody 11026-1-AP	Download protocol
FC protocol for CDK4 antibody 11026-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

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