BD Biosciences/APC Mouse Anti-Human CD5/5 μL/340583_蚂蚁淘，【正品极速】生物医学科研用品轻松购|ebiomall 蚂蚁淘商城

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BD Biosciences/APC Mouse Anti-Human CD5/5 μL/340583

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BD Biosciences/APC Mouse Anti-Human CD5/5 μL/340583

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Bacteria

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340583

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Product DetailsRecommended AssayReferences

Description

CD5, clone L17F12, is derived from hybridization of mouse NS-1/Ag4 myeloma cells with spleen cells from BALB/c mice immunized with human T-acute lymphoblastic leukemia (ALL) cells.

CD5 recognizes a human T-lymphocyte antigen, 67 kilodaltons (kDa).

Format

FormatAPC
Excitation SourceRed 633 nm
Excitation Max650nm
Emission Max660nm

Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously

Resources & Tools
Spectrum Viewer	Panel Designer	SpectrumViewer	Download TDS	Download MSDS

Preparation and Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

Antin JH, Emerson SG, Martin P, Gadol N, Ault KA. Leu-1⁺ (CD5⁺) B cells. A major lymphoid subpopulation in human fetal spleen: phenotypic and functional studies. J Immunol. 1986;136:505-510.
Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R. Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. Proc Natl Acad Sci USA. 1981;78:1791-1795.
Gadol N, Ault KA. Phenotypic and functional characterization of human Leu1 (CD5) B cells. Immunol Rev. 1986;93:23-24.
Hardy RR, Hayakawa K, Shimizu M, Yamasaki K, Kishimoto T. Rheumatoid factor secretion from human Leu-1⁺ B cells. Science. 1987;236:81-83.
Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. New York, NY: Springer-Verlag; 1986:259-288.
Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T-lymphocyte helper/inducer and T cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981;153:310-323.
Ledbetter JA, Frankel AE, Herzenberg LA. Human Leu T-cell differentiation antigens: quantitative expression on normal lymphoid cells and cell lines. In: Hämmerling G, Hämmerling U, Kearney J, eds. Monoclonal Antibodies and T-Cell Hybridomas: Perspectives and Technical Advances. New York, NY: Elsevier/North Holland; 1981:16-22.
Royston I, Majda JA, Baird SM, Meserve BL, Griffiths JC. Human T-cell antigens defined by monoclonal antibodies: the 65,000-dalton antigen of T cells (T65) is also found on chronic lymphocytic leukemia cells bearing surface immunoglobulin. J Immunol. 1980;125:725-731.
Warnke R, Levy R. Detection of T and B cell antigens hybridoma monoclonal antibodies: a biotin-avidin-horseradish peroxidase method. J Histochem Cytochem. 1980;28:771-776.
Warnke R, Miller R, Grogan T, Pederson M, Dilley J, Levy R. Immunologic phenotype in 30 patients with diffuse large-cell lymphoma. N Eng J Med. 1980;303:293-300.
Zipf TF, Fox RI, Dilley J, Levy R. Definition of the high-risk acute lymphoblastic leukemia patient by immunological phenotyping with monoclonal antibodies. Cancer Res. 1981;41:4786-4789.

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