BD Biosciences/Purified Mouse Anti-Human CD16/20 μL/347530_蚂蚁淘，【正品极速】生物医学科研用品轻松购|ebiomall 蚂蚁淘商城

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BD Biosciences/Purified Mouse Anti-Human CD16/20 μL/347530

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BD Biosciences/Purified Mouse Anti-Human CD16/20 μL/347530

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Bacteria

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347530

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4000-520-616

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Product DetailsRecommended AssayReferences

Description

CD16 (Leu-11a), clone NKP15, is derived from hybridization of mouse P3-X63-Ag8 myeloma cells with spleen cells from BALB/c mice immunized with large granular lymphocytes.

CD16 (Leu-11b), clone GO22, is derived from hybridization of mouse P3-X63 myeloma cells with spleen cells from BALB/c mice immunized with granulocytes.

CD16 (Leu-11c), clone B73.1, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with NK lymphocytes.

CD16 antibodies recognize an Mr 50- to 65-kilodalton (kd) antigen present on human natural killer (NK) lymphocytes that is the IgG Fc receptor III.

Format

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Preparation and Storage

The monoclonal antibody is supplied as 100 μg purified immunoglobulin in 2.0 mL (50 μg/mL) of phosphate -buffered saline (PBS). The FITC conjugate is supplied as 250 μg in 2.0 mL (125 μg/mL) of PBS. The PE conjugate is supplied as 50 μg in 2.0 mL (25 μg/mL) of PBS. The PE-Cy7 conjugate is supplied as 50 μg in 0.5 mL (100 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

Campbell JJ, Qin S, Unutmaz D, et al. Unique subpopulations of CD56⁺ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire. J Immunol. 2001;166:6477-6482.
Cooper MD, Fehniger TA, Caligiuri MA. The biology of human natural killer–cell subsets. Trends in Immunol. 2001;22:633-640.
Galandrini R, Tassi I, Mattia G. SH2-containing inositol phosphatase (SHIP-1) transiently translocates to raft domains and modulates CD16-mediated cytotoxicity in human NK cells. Blood. 2002;100:4581-4589.
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Lanier LL, Kipps T, Phillips JH. Functional properties of a unique subset of cytotoxic CD3⁺ T lymphocytes that express Fc receptors for IgG (CD16/Leu-11 antigen). J Exp Med. 1985;162:2089.
Lanier LL, Le AM, Civin CI, Loken MR, Phillips JH. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol. 1986;136:4480-4486.
Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983;131:1789-1796.
Perussia B, Acuto O, Terhorst C, et al. Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. II. Studies of B73.1 antibody-antigen interaction on the lymphocyte membrane. J Immunol. 1983;130:2142-2148.
Perussia B, Starr S, Abraham S, Fanning V, Trinchieri G. Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. I. Characterization of the lymphocyte subset reactive with B73.1. J Immunol. 1983;130:2133-2141.
Perussia B, Trinchieri G, Jackson A, et al. The Fc receptor for IgG on human natural killer cells: Phenotypic, functional, and comparative studies with monoclonal antibodies. J Immunol. 1984;133:180-189.
Phillips JH, Babcock GF. A monoclonal antibody reactive against purified human natural killer cells and granuocytes. Immunol Letters. 1983;6:143.
Schmidt RE. Non-lineage/natural killer section report: new and previously defined clusters. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Osford University Press; 1989:517-542.
Thompson J, Goeken N, Brown S, Rhodes J. Phenotypic definition of human monocyte/macrophage subpopulations by monoclonal antibodies. II. Distinction of non-T, non-B natural killer cells (NK) from antigen-presenting stimulating cells in the mixed lymphocyte response (MLR). Presented at the American Association for Clinical Histocompatibility Testing.

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