Description
CD34 (Anti–HPCA-1), clone My10, is derived from the hybridization of mouse Sp2/0-Ag14 cells with spleen cells of BALB/c mice immunized with the human cell line KG-1a.
CD34 (Anti–HPCA-2), clone 8G12, is derived from the hybridization of mouse Sp2/0-Ag14 cells with spleen cells of BALB/c mice immunized with the human cell line KG-1a.
The CD34 antigen is a single-chain transmembrane glycoprotein, Mr 105 to 120 kilodaltons (kd). The antigen is associated with human hematopoietic progenitor cells and is a differentiation stage–specific leucocyte antigen. Clone My10 and clone 8G12 recognize two distinct CD34 epitopes; at least three epitopes have been identified.
Format
- FormatPE
- Excitation SourceBlue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max496nm
- Emission Max578nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
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Preparation and Storage
Clone My10: The monoclonal antibody is supplied as 100 µg purified immunoglobulin in 2.0 mL (50 µg/mL) of phosphate-buffered saline (PBS).
Clone 8G12: The monoclonal antibody is supplied as 50 µg purified immunoglobulin in 2.0 mL (25 µg/mL) of PBS. The FITC and PE conjugates are each supplied as 50 µg in 2.0 mL (25 µg/mL) of PBS. The PerCP conjugate is supplied as 50 µg in 1.0 mL (50 µg/mL) of PBS. The PerCP-Cy5.5 conjugate is supplied as 12.5 µg in 1.0 mL (12.5 µg/mL) of PBS. The PE-Cy7 conjugate is supplied as 25 µg in 0.5 mL (50 µg/mL) of PBS. The APC conjugate is supplied as 50 µg in 0.5 mL (100 µg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide. Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
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